摘要
本文建立了人血浆中格列苯脲的高效液相色谱-质谱/质谱(HPLC-MS/MS)分析方法.血浆中格列苯脲测定的线性范围是1~300 ng/mL;高、中、低三个浓度分析测定的精密度(RSD)<15%;格列苯脲和格列齐特(内标)绝对回收率均>80%.本方法灵敏、准确,重现性好,用于血药浓度的测定获得满意结果.
A sensitive high performance liquid chromatographic method coupled with tandem mass spectrometry (HPLC-MS/MS) for the determination of glibenclamide in human plasma was developed. The standard curve was linear (r≥0. 999) over the glibenclamide concentration range of 1-300 ng/mL for glibenclamide. The intra-assay and inter-assay precision of the assay were within 15% relative standard deviation. The extraction recoveries of glibenclamide and internal standard were above 80%. The present method has been successfully applied to determine the concentration of glibenclamide in plasma.
出处
《分析科学学报》
CAS
CSCD
2007年第3期280-282,共3页
Journal of Analytical Science