高效液相色谱-质谱/质谱测定血浆中格列苯脲

Determination of Glibenclamide in Human Plasma by HPLC-MS/MS

本文建立了人血浆中格列苯脲的高效液相色谱-质谱/质谱（HPLC-MS/MS）分析方法.血浆中格列苯脲测定的线性范围是1～300 ng/mL;高、中、低三个浓度分析测定的精密度（RSD）＜15%;格列苯脲和格列齐特（内标）绝对回收率均＞80%.本方法灵敏、准确,重现性好,用于血药浓度的测定获得满意结果.

A sensitive high performance liquid chromatographic method coupled with tandem mass spectrometry （HPLC-MS/MS） for the determination of glibenclamide in human plasma was developed. The standard curve was linear （r≥0. 999） over the glibenclamide concentration range of 1-300 ng/mL for glibenclamide. The intra-assay and inter-assay precision of the assay were within 15% relative standard deviation. The extraction recoveries of glibenclamide and internal standard were above 80%. The present method has been successfully applied to determine the concentration of glibenclamide in plasma.