槐定碱致痫大鼠海马清醒脑电图及血清细胞因子含量变化的研究

Study of the Change of Serum Cytokines and Hippocampal Electroencephalograph in Waking State of Rats with Epilepsy Induced by Sophocarpine(槐定碱)

目的:建立槐定碱(sophojridine,SRI)诱导大鼠癫痫模型,同步观察槐定碱致痫大鼠行为和清醒脑电的变化,并探求细胞因子与癫痫发病之间的关系。方法:选取30只大鼠,通过立体定位仪在大鼠海马埋置电极,1周后将存活健康的26只大鼠随机分成对照组和实验组,实验组大鼠予腹腔注射槐定碱(55mg/kg),造成强直-阵挛性发作癫痫持续状态模型;对照组腹腔注射等量生理盐水,观察记录自由大鼠海马的脑电活动和行为学改变;同时运用放射免疫法,检测大鼠血清TNF-α、IL-2和IL-6的水平。结果:实验组大鼠腹腔注射槐定碱后,逐渐出现癫痫样发作,且海马脑电图同时显示癫痫波发放,对照组大鼠无癫痫症状且未记录到痫性脑电图;实验组大鼠血清中TNF-α、IL-2和IL-6含量均明显高于对照组(P<0.01)。结论:大剂量槐定碱腹腔注射可使大鼠引发典型的痫样发作行为和痫样脑电;TNF-α、IL-2、IL-6在槐定碱所致的癫痫发病中起促进作用,是癫痫发病的基础之一。

Objective：To make the rat model of epilepsy with sophocarpine, to observe the behavior and electroencephalograph in waking state of rats. And to study the correlation of cytokines and epilepsy. Methods ： electrodes were implanted in the hippocampal gyrus of rats with stereotaxic instrument. 1 week later, 26 rats were randomly divided into the control group and the experiment group. Rats in the experiment group were given high dose of sophocarpine through intra-abdominal injection to make the model of tonoclonic epilepticism. And normal saline was given to the control group. Hippocampal electroencephalograph in waking state, behavior of rats and the content of serum TNF-α,IL-2 and IL-6 were detected . Results. In the experiment group, epileptic attack occured after given sophocarpine. The brain wave of epilepsy emerged in the hippocampal electroencephalograph. Serum TNF-α,IL-2 and IL-6 were much higher than the control group （P 〈 0. 01 ）. Conclusion. High dose of sophocarpine can induce epileptic attack and epileptic electroencephalograph. TNF-α,IL-2 and IL-6 play an important role in epilepsy induced by sophocarpine.