复方八正散液灌胃大鼠尿液的体外抑菌和对左氧氟沙星增效作用的实验研究

Experimental Study on the In Vitro Bacteriostatic Effect and Synergic Effect on Levofloxacin of the Urine of Rats Lavage with Compound Bazhensan Liquid (CBL,复方八正散液)

目的:观察复方八正散液灌胃大鼠尿液的体外抑菌作用和对左氧氟沙星抗菌效果的增效作用。方法:大鼠给药后收集尿液,采用微量二倍稀释法和平板转染法测定复方八正散液灌胃大鼠尿液对尿路感染常见致病菌的最小抑菌浓度(MIC)、最小杀菌浓度(MBC)和对左氧氟沙星抗菌效果的增效作用。结果:复方八正散液灌胃大鼠尿液体外能有效抑制和灭活大肠埃希菌、金黄色葡萄球菌、表皮葡萄球菌、变形杆菌,与左氧氟沙星合用,增加后者的抗菌效果稀释度。结论:复方八正散液能抑制和灭活尿路感染常见致病菌,对左氧氟沙星的抗菌效果具有增效作用。

Objective： To observe the in vitro bacteriostatic effect and synergic effect on levofloxacin of the urine of rats lavage with compound Bazhensan liquid. Methods ： The urine of rats were collected after given CBL. Minimal inhibitory concentration （MIC） and minimal bactericidal concentration （MBC） of common bacteria of urinary tract infection and the synergic effect on levofloxacin of the urine were detected with the methods of micro-doubling dilution and plating transfection. Results ： The urine of rats lavage with CBL can inhibit the growth of Escherichia coli, Staphylococcus aureus and Staphylococcus epidermidis in vitro. It can also increase the dilution titer of levofloxacin. Conclusion： CBL can inhibit the common bacteria of urinary tract infection effectively. And it has synergic effect on levofloxacin.