三种肾脏病人血浆中抗碳酸酐酶IV抗体检测方法的建立

Method for the detection of plasma anti-CA IV antibody in kidney diseases

目的建立使用间接酶联免疫吸附法(ELISA)检测人血浆抗碳酸酐酶IV抗体的方法,阐明血浆抗碳酸酐酶IV抗体滴度与肾病综征合(NS)、慢性肾小球肾炎(CGN)和2-型糖尿病肾病(2-DN)三种肾脏病的联系。方法建立并使用间接ELISA法检测三种肾脏病人血浆中抗碳酸酐酶IV抗体的滴度。结果成功建立使用间接ELISA检测人血浆抗碳酸酐酶IV抗体的方法,其特异性、稳定性和精密度均可靠。CGN和2-DN病人血浆抗碳酸酐酶IV抗体的滴度均显著高于对照(P<0.05),NS病人血浆抗碳酸酐酶IV抗体的滴度与对照无统计学差异(P>0.05)。结论用间接ELISA法检测人血浆抗碳酸酐酶IV抗体是可行的。在CGN和2-DN中,IV型碳酸酐酶可能是靶抗原,说明IV型抗碳酸酐酶抗体在肾脏疾病体液免疫方面的发生发展中起到了重要作用。

Objective To develop a indirect ELISA method for the detection of plasma anti - CA Ⅳ antibody and to evaluate the relationship between anti - CA Ⅳ antibody with pathogenesis of NS, CGN and 2 - DN. Methods An attempt was made to develop an indirect ELISA method for the detection of plasma anti - CA Ⅳ antibody, and it was used to detect plasma anti - CA Ⅳ antibody in patients with three kinds of kidney diseases. Results An indirect ELISA method for the detection of plasma anti - CA Ⅳ antibody was successfully developed with favorable specificity, stability and precision. Plasma anti - CA Ⅳ antibody titer was significantly elevated in patients with CGN and 2 - DN （ P 〈 0.05 ）, plasma anti - CA Ⅳ antibody titer in patients with NS was similar to that of control group （ P 〉 0.05 ）. Conclusion It is feasible to develop a indirect ELISA method for the detection of plasma anti - CA Ⅳ antibody. CA Ⅳ may be target antigen in CGN and 2 - DN and anti - CA Ⅳ antibody may play an important role in the pathogenesis of humoral immunity of renal diseases.