脑胶质瘤中脆性组氨酸三联体和PTEN的表达

Expressions of FHIT and PTEN in Brain Gliomas

目的检测抑癌基因脆性组氨酸三联体(FHIT)、PTEN在胶质瘤中的表达,并探讨其与肿瘤恶性程度的关系。方法采用免疫组织化学SP法,检测80例胶质瘤、5例正常脑组织中的FHIT、PTEN蛋白的表达。结果FHIT主要定位于肿瘤细胞的细胞质,PTEN主要定位于肿瘤细胞的细胞核,总阳性率分别为47.5%(38/80)、55.0%(44/80);FHIT和PTEN在正常脑组织中表达率均为80%(4/5),两者在正常脑组织和不同级别胶质瘤之间的表达均有差异(P<0.05),且随胶质瘤恶性程度的增加而减弱。结论FHIT和PTEN在胶质瘤细胞中表达减少或缺失,两者协同作用促进胶质瘤的发生发展。

Objective To examine the expressions of FHIT and PTEN in gliomas, and to investigate the relationship between their expressions and the degree of tumor malignancy. Methods SP Immunohistochemical staining was used to examine the expressions of FHIT, PTEN in 80 gliomas and in 5 normal brain tissues. Results FHIT was located in the plasma of glioma cells,PTEN was located in the cellular nuclei of glioma cells, total positive rates were 47. 5% （38/80） ,55.0% （44/80）respectively. Expression rates of FHIT and PTEN in normal brain tissues were 80% （4/5）. There was a statistically significant difference in the expression of FHIT and PTEN between normal brain tissues and different grades of gliomas （P〈0. 05）. Both of the expressions of FHIT and PTEN weakened with the rise in the degree of glioma malignancy. Conclusion Expressions of FHIT and PTEN in glioma cells are diminishing or absent, their cooperative operation promotes generation and advance of glioma.