肝素对小鼠肝癌细胞Hca-F和Hca-P淋巴道转移的抑制作用

Inhibitory Effects of Heparin on Mouse Hepatocarcinoma Cell Line Hca-F and Hca-P's Lymphogenous Metastasis

目的观察肝素对小鼠肝癌高转移株Hca-F和低转移株Hca-P细胞与冰冻淋巴结切片体外粘附和体内淋巴道转移的影响,探讨其作用的分子机制。方法采用细胞黏附检测(Stamper-WoodruffMethod)、组织化学、流式细胞分析等方法,观察和检测肿瘤细胞粘附和转移的情况。结果肝素体外对小鼠肝癌细胞Hca-F和Hca-P与冰冻淋巴结切片粘附有明显的抑制作用,100U、150U肝素组与对照组比较有高度统计学意义(P<0.01);肝素体内对小鼠肝癌细胞Hca-F淋巴道转移也有明显的抑制作用,100U肝素组与对照组比较有统计学意义(P<0.05)。结论小鼠肝癌细胞Hca-F和Hca-P均有L-选择蛋白的表达,仅程度不同,L-选择蛋白的表达水平高低与Hca-F和Hca-P细胞的淋巴道转移潜能正相关,肝素竞争性抑制L-选择蛋白与其配体的结合过程,从而达到抑制肿瘤细胞转移的目的,这可能是肝素抑制肿瘤及其转移作用的分子机制之一,由此也说明L-选择蛋白介导和协助了Hca-F和Hca-P细胞的淋巴道转移。

Objective To observe the effects of heparin on mouse hepatocarcinoma cell line Hca-F and Hca-Ps adhesion in vitro and the lymphogenous metastasis in vivo. In order to detect bow heparin plays its role. Methods Using Stamper-Woodruff method, cell chemistry and flow cytometry analysis to detect the effects of heparin in different concentrations. Results Heparin can inhibit obviously the adhesion between Hea-F and Hca-P cells and ice frozen sections of mouse lymph nodes in vitro, compared with saline-treatment group（P〈0. 01 ） ; heparin can inhibit the lymphogenous metastasis of Hca-F cells in vivo, which has statistical significance between 100 U heparin group and saline-treatment group （P〈0.05）. Conclusion Both Hca-F and Hca-P cells have the expression of L-selectin, but their expression level is different. The expression level of L-selectin on mouse hepatocarcinoma cell line Hca-F and Hca-P is positively related to the lymph node metastasis potential. Interaction between L selectin and its ligand is inhibited partially by heparin. Therefore heparin have the competence of inhibiting tumor cell＇s lymphogenous metastasis. Meanwhile, it also demonstrates that L-selectin can facilitate metastasis of Hca-F and Hea-P cells to the lymph node.