DNA双链断裂修复蛋白在宫颈癌和CIN组织中的表达

Expression of DNA Double-strand Break Repair Protein in Cervical Carcinoma and CIN Tissue

目的研究DNA双链断裂修复蛋白(Ku80、DNA-PKcs和ATM)在宫颈癌和宫颈上皮内瘤样病变(CIN)组织中的表达情况,探讨3种蛋白在宫颈癌发生发展中的作用及相互关系。方法应用免疫组化SP法检测41例宫颈癌组织和15例CIN组织中Ku80,DNA-PKcs和ATM蛋白的表达情况。结果Ku80、DNA-PKcs和ATM蛋白在宫颈癌患者中的阳性率分别为70.7%、68.3%和19.5%,在CIN患者中的阳性率分别为80.0%、73.3%和33.3%;3种蛋白在宫颈癌组织中的表达均低于CIN组织,但差异无统计学意义(P>0.05)。3种蛋白的表达在不同年龄、病理类型、分化程度和临床分期的宫颈癌患者中差异无统计学意义(P>0.05)。Spearman等级相关分析,在56例患者中,Ku80与DNA-PKcs的表达呈正相关(r=0.583,P=0.000);Ku80与ATM的表达亦呈正相关(r=0.274,P=0.041)。结论3种蛋白可能未在宫颈癌的发生发展中起主要作用;Ku80与DNA-PKcs及与ATM之间存在密切关系。

Objective To study the expression of DSB repair protein （including Ku80, DNA-PKcs and ATM） in cervical carcinoma and cervical intra-epithelial neoplasia （CIN）, and to explore their roles in neoplasia and tumor progression of cervical carcinoma. Methods Immunohistochemistry was used to detect the expressions of Ku80, DNA-PKcs, and ATM in 41 cases of cervical carcinoma and 15 cases of CIN. Results The positive percentages of Ku80, DNA-PKcs and ATM were 70. 7% ,68. 3% and 19. 5% respectively in cervical carcinoma, and were 80. 0%, 73.3% and 33.3% respectively in CIN; the expression of three proteins in CIN were all higher than that in cervical carcinoma, but the differences has no statistical significance （P〉0. 05）. The expression of these proteins was not associated with the age of patients, pathology, differentiation, and clinical stage （P〉0. 05）. In all 56 cases, Ku80 was positively correlated with DNA-PKcs （r= 0. 583,P= 0. 000） and ATM （r= 0. 274,P= 0. 041）. Conclusion Three proteins might not play the major role in the neoplasia and tumor progression of cervical carcinoma; Ku80 had closely relationship with DNA-PKcs and ATM in cervical carcinoma and CIN.