低强度脉冲超声波对β-磷酸三钙与兔骨髓基质干细胞相容性的影响

Effect of low intensity pulsed ultrasound on biocompatibility between β-tricalcium phosphate and rabbit bone mar-row stromal cells

目的研究低强度脉冲超声波(LIPUS)对-磷酸三钙(-TCP)与兔骨髓基质干细胞(BMSCs)相容性的影响。方法抽取新西兰白兔的骨髓,分离纯化获取BMSCs,加入条件培养基和-TCP体外混合培养并随机分为两组,一组应用LIPUS对BMSCs每天作用20分钟,另一组作为对照未予作用。通过倒置相差显微镜观察细胞增殖分化情况,分别于1周、2周、3周后停止LIPUS作用,在扫描电镜下观察细胞生长情况,评估TCP与BMSCs的相容性。结果BMSCs在两组-TCP表面生长繁殖良好。混合培养1周、2周时,实验组TCP表面BMSCs数目较对照组明显增多,并具有统计学意义(<0.05)。混合培养3周时,实验组TCP表面BMSCs数目与对照组相比无统计学差异(﹥0.05)。结论LIPUS可以提高培养早期-TCP和BMSCs的生物相容性,而对于培养晚期TCP和BMSCs的生物相容性无明显影响。

Objective To study the effect of low intensity pulsed ultrasound （LIPUS） onthe biocompatibility between β-tricalcium phosphate （TCP） and rabbit bone marrow stromal cells （BMSCs）. Methods Some bone marrow of a rabbit were drown out and cultured by differentiation culture medium to harvest BMSCs and the third gefieration BMSCs were randomly assigned to the experimental groups and the control groups. The morphology of BMSCs in the experimental groups, which stimulated by LIPUS 20min/day for one week, two weeks, or three weeks, and the control groups were all observed with phase-contrast microscope and scanning electron microscope. Results BMSCs could fully attach to and extend to TCP, and excreted extracellular matrix. The number of BMSCs on the experimental group increased significantly compared with the control groups at the first week and second week （P 〈 0.05）. But the number of BMSCs on the experimental group increased significantly compared with the control groups at the third week. LIPUS stimulation upregulated the biocompatibility between TCP and BMSCs compared to the control group at early stage and have not same obvious effect at late stage. Conclusion LIPUS stimulation upregulated the biocompatibility between TCP and BMSCs compared to the control group at early stage and have not same obvious effect at late stage.