Fischer 344大鼠泌乳素腺瘤动物模型的建立

Establishment of Animal Model of Prolactinoma with Fischer 344 Rats

目的建立Fischer344(F344)大鼠泌乳素腺瘤动物模型。方法采用F344雌性大鼠,行腹腔注射苯甲酸雌二醇或无菌生理盐水,用药50d后行MRI扫描,观察大鼠生存状态,检测垂体重量、血清泌乳素(PRL)水平、病理与PRL免疫组织化学检测变化。结果应用苯甲酸雌二醇50d后,大鼠体重增长受抑,垂体重量与PRL水平显著升高,病理检查可见垂体肿瘤形成且PRL染色阳性,MRI可见肿瘤,成瘤率达100%。结论利用F344大鼠建立的垂体瘤动物模型,成瘤周期短,简单易行,稳定可靠,符合泌乳素腺瘤的生物学特性。

Objective To establish animal model of prolactinoma with Fischer 344 （F344） rats. Methods Female F344 rats were injected with estradiol henzoate-treated and 0.9 % sodium chloride respectively. After 50 days of treatment, MRI scan was performed and animal＇s living status was observed, and pituitary weight, serum prolactin level, pathological and PRL immunohistochemistry changes were also detected. Results All estradiol benzoate-treated rats formed the prolactinoma after treatment for 50 days and the growth of body weight was retarded obviously. Pituitary weight and serum PRL increased significantly in estradiol benzoate-treated animals compared with those treated with 0.9% sodium chloride. Pituitary adenomas were found in MRI scan and HE stain was positive. The forming rate of tumor was 100%. Conclusion The animal model of pituitary adenoma established with F344 rat has more characters such as having a short tumor forming period, easy and stable, and coincided with features of prolactinoma.