65株变形杆菌产β-内酰胺酶的类型与分布情况研究

Studies on the classification and distribution of the β-Lactamases in 65 strains of Proteus spp

目的了解65株变形杆菌产β-内酰胺酶（β-lase）的类型与分布情况。方法采用多底物改良相邻纸片法检测65株变形杆菌（奇异变形杆菌37株，普通变形杆菌15株，其它变形杆菌13株）所产β-lase的类型与分布情况。结果①65株变形杆菌β-lase总检出率为44．6％，其中青霉素酶6株（9．2％），头孢菌素酶7株（10．8％），而检出产广谱酶13株（20．0％），超广谱D-内酰胺酶（ESBLs）3株（4．6％）；②不同变形杆菌的产酶不同，奇异变形杆菌以产广谱酶为主，普通变形杆菌以产头孢菌素酶为主，其它变形杆菌以产超广谱酶为主。结论①本组变形杆菌产β-lase以广谱酶、头孢菌素酶为主；②不同变形杆菌的主要酶型不同。

Objective To study the classification and distribution of the β-Lactamases in 65 strains of Proteus spp. Methods To detect the β-Lactamases produced by Proteus spp. （P.mirabilis 37 strains, Pvulgaris 15 strains, other types of Proteus spp. 13 strains） with a multi-disk synergy test. Results （1） 29 strains of 65 strains produced the β-Lactamases, total producing rate was 44.6%, penicillinases 6 strains （9.2%）, cephalosporinases 7 strains （10.8%）, 13 strains producing broad-spectrum β-Lactamases （BSBLs, 20.0%）; 3 strains produced extented-spectrum β-Lactamases （ESBLs, 4.6%）; all strains did not produce carbapenem-hydrolyzing β-Lactamases （CHBLs）; （2） There were different β-Lactamases produced by different types of Proteus spp.. The main type of β-Lactamases produced by P. mirabilis was the BSBLs, the main type of β-Lactamases produced by P. vulgaris was the cephalosporinases, and the main type of β-Lactamases produced by other types of Proteus spp. was ESBLs. Conclusions （1） The types of β-Lactamases produced by the 65 strains of Proteus spp. was mainly BSBLs and cephalosporinases, all strains did not produce CHBLs; （2） There were different β-Lactamases produced by different types of Proteus spp.