中药干预偏头痛大鼠脑膜核因子NF-кB mRNA表达的影响

The Intervention Effect of Traditional Chinese Medicine on Meningeal Nuclear Factor Expression of Rats Suffering from Migraine

探讨了中药干预偏头痛大鼠脑膜核因子的机制。选用成年SD大鼠,采用Cristina tassorelli方法制备偏头痛模型。分为正常组、模型组和中药组,后二组又分为0.5、1.5、2.5、4h4个间隔点,以模型组做对照。运用实时荧光定量RT-PCR技术检测大鼠脑膜核因子的基因表达。结果表明:0.5h组、1.5h组和2.5h组与模型组相比,P>0.05;4h组与模型组相比,P<0.05,有统计学意义。中药干预偏头痛模型大鼠脑膜核因子可不同程度地抑制异常升高的脑膜核因子NF-кB mRNA的表达,说明中药干预可能是通过抑制脑膜NF-кB基因的过度表达而使颅脑血管舒缩系统障碍得到改善与治疗。该结论为研制以NF-кB为靶点的中药提供理论依据及治疗时间窗,并为临床治疗偏头痛提供深层次的客观依据。

This animal experiment was made to probe into the mechanism by which traditional Chinese medicine interferes with the meningeal nuclear factor of rats suffering from migraine. The adult SD rats were selected, and the Cristina Tassorelli method was adopted for the preparation of the migraine models, which were classified into the normal group, model group and traditional Chinese medicine group respectively, and the latter two groups were further divided, in terms of intervals, into 0. 5 h, 1.5 h, 2. 5 h and 4 h groups, with the model group as control. Real-time quantitative RT-PCR technology was employed to test and measure the gene expression of meningeal nuclear factor of rats. The results showed that when the level of NF-κB mRNA expression in the model group was compared with that in 0. 5h group, 1.5h group and 2.5h group, there was no significant difference （P〉0. 05）, but when compared with that in the 4h group, there was significant difference （P〈0. 05）, Intervention by traditional Chinese medicine in the gene expression of meningeal nuclear factor of model rats suffering from migraine can restrain at different degree the expression of abnormally increasing miningeal nuclear factor NF-κB mRNA, which suggests that the intervention by traditional Chinese medicine may enable improvement and treatment of the obstacle of the constriction and dilation system of cerebral blood vessels through restraining excessive expression of meningeal NF-κB genes. This conclusion could serve as a theoretical basis for developing traditional Chinese medicine, which takes the NF-κB as the target point and the treatment time window, and have underpins an objective approach to the clinical treatment of migraine.