纳米拓扑结构的构建以及对细胞行为的影响

Effects of Nanotopography for Biomaterials on Cell Behaviors

生物材料的拓扑形貌是影响细胞行为的重要因素之一,近年来随着纳米生物和医学技术的进展,研究发现细胞能够通过“接触诱导”感应基底材料的纳米拓扑结构,产生强烈的响应。我们对纳米结构的不同构建方法进行了综述,分析不同的纳米拓扑结构对不同种类细胞及其功能的作用,包括对细胞黏附、增殖、分化、凋亡以及细胞外基质的重建等行为的影响和可能的发生机制,探讨了纳米拓扑结构的构建对于从分子和细胞水平上控制生物材料与细胞间的相互作用,从而引发特异性细胞反应的重要作用,以及对于组织再生与修复的潜在应用前景和意义。

The topography structure of biomaterial is one of the most important factors which affect cells＇behaviors, including cell adhesion, proliferation, differentiation and apoptosis. With the development of nanobiology and technology, it is well known that cells could sense and respond to the nanotopography of material＇s substratum with ＂contact guidance＂, and the nanotopography of the substratum has potential application and significance to tissue regeneration and rehabilitation. In this article, various methods to constitute nanotopography including polymer demixing, electrospinning, electron beam lithography as well as chemical grafting were reviewed, the effects of nanotopography on cells＇growth behavior and reconstruction of extracellular matrix were analyzed, and the important functions of nanotopography construction to the cell-material interaction at the molecular and cellular level were discussed.