用猴艾滋病D型逆转录病毒筛选抗艾滋病药物实验模型的建立及应用

Establishment and Application of an Experimental Model for ScreeningAnti-AIDS Drugs with Simian Type D Retrovirus

以药物对合胞体形成的抑制和对细胞的毒性作为检测指标，应用猴艾滋病D型逆转录病毒（SRV）／Raji细胞系统建立了体外筛选抗艾滋病药物的实验模型。AZT和天花粉蛋白可显著地抑制SRV诱导的合胞体形成，它们的选择指数（SI）分别为数13500和8812。用该模型筛选了46种来源于天然资源的化合物，结果表明有11种天然化合物有明显的抗病毒活性。

Because of the close phylogenetic relationship, the simian acquired immunodeficiency syndrome (SAIDS) is considered as an important model in search of new drugs in prevention and treatment of the pandemic human AIDS. The simian AIDS type D retroviruses (SRV) and simian immunodeficiency viruses (SIV) are the causative agents of SAIDS. A SRV model to screen anti -AIDS drugs in vitro was established. AZT and trichosanthin (TCS) were shown to inhibit SRV1 syncytium formation obviously. The selectivity indexes (SI) of AZT and TCS were 13500 and 8812, respectively. Forty-six natural compounds isolated from the Chinese medical herbs were screened by using this model. There were eleven natural compounds with obvious antiviral activity in vitro. These data indicated that SRV model for screening anti-AIDS drugs was feasible.