激光对尾草履虫分裂繁殖影响的研究

The effect of Laser on Division and Reproduction of Paramecium Caudatum 

采用Ｈｅ—Ｎｅ激光辐照经单克隆培养的尾草履虫（Ｐａｒａｍｅｃｉｕｍｃａｕａｔｕｍ）无性繁殖系，观察并分析激光对尾草履虫分裂繁殖产生的影响。结果表明，在本实验条件下，当辐照的能量密度处于５０．９—１０１．９（Ｊ／ｃｍ２）范围内，激光对尾草履虫的分裂繁殖产生促进作用。当辐照的能量密度为１０１．９Ｊ／ｃｍ２时，草履虫分裂繁殖的个体平均数达最大值。草履虫对激光辐照有很大的耐受范围和很强的耐受能力。本实验采用的辐照剂量范围宽（从２５．５Ｊ／ｃｍ２至１５２．８Ｊ／ｃｍ２），辐照剂量大（最高达１５２．８Ｊ／ｃｍ２），但对草履虫分裂繁殖表现为起促进作用或无不利影响。这一结果说明，最原始、最低等的单细胞动物对激光有强的耐受性；同时，从对光的反应方面。

Paramecium caudatum(P.c.)cultured by clone was treated with He—Ne laser.The results showed irradiation promoted the division and reproduction of P.c. when the dosages of laser were between 50.9 J/cm 2 and 101.9 J/cm 2 .The most proper dosage was 101.9 J/cm 2 under the condition of this experiment. The speed was accelerated or,at least,no disadvantage effect on division and reproduction of P.c.under very intensive and very wide range of dosages from 25.5J/cm 2 to 152.8J/cm 2.The results indicated the lowest and the most original unicellular animals had very intensive tolerance to laser.It also provided relevant data for research in ecology of protozoa in respect of light.