DAB诱发大白鼠肝癌过程中细胞质膜蛋白质双向电泳的研究

Study of Two-dimensional Gel Electrophoresis in The Protein Pattern of Plasma Membrane from Rat Livers During Hepatocarcinogenesis Induced by DAB

本实验以二甲基氨基偶氮苯（ＤＡＢ）诱发大白鼠肝癌的动物模型为材料，用电脉方法观察了在诱癌过程中和肝癌形成后大白鼠肝细胞质膜上蛋白质的变化。肝细胞质膜蛋白的ＳＤＳ—ＰＡＧＥ电泳图谱显示：在诱癌４周、１６周的肝细胞和肝细胞癌的细胞质膜蛋白与正常肝比较没有显著差异。胆管癌细胞质膜蛋白的ＳＤＳ—ＰＡＧＥ电泳图谱与正常肝细胞比较，在１５．８ＫＤ～６９．２ＫＤ的带群中新出现一条深染的蛋白带。用双向电泳方法对肝细胞质膜蛋白进行分析，诱癌４周和１６周的肝细胞质膜蛋白双向电泳图谱与正常肝比较，除了等电点低于ｐＨ６．８４的蛋白质点数略有增加外，没有显著差异。在肝细胞癌质膜蛋白双向电泳图谱上，等电点高于ｐＨ７．８３的蛋白质点数略有减少，等电点低于ｐＨ６．

We had observed the changes of protein in plasma membranes during DAB induced rat hepatocarcinogenesis by gel electrophoresis method.The results revealed are as follows: In the SDS-PAGE patterns of hepatic plasma membrane proteins,no difference was found among the normal livers,hapatomas and livers of 4 weeks and 16 weeks after DAB administration,but a new protein band was found between 15.8KD-69.2KD band groups from the cholangiocarcinoma plasma membrane protein. The patterns of 2-D PAGE of plasma membrane proteins showed 310 spots in normal rat liver,305 and 301 spots in liver of 4 weeks and 16 wddks after DAB administration and 298 spots in hepatocarcinoma.There is no significant difference between normal liver and livers of 4 weeks and 16 weeks after DAB administration.Comparing with normal liver,the pattern of 2-DPAGE of the hepatocarcinoma shows that the proteins over pH7.83 decreased