摘要
以0.05mol/L硫酸作对照液,用紫外分光光度法在421nm测定了盐酸环丙沙星、盐酸小檗碱预混剂中盐酸小檗碱的含量。结果表明,盐酸小檗碱在8-56μg/mL范围内,吸光度与浓度呈良好的线性关系,相关系数为0.9992,平均回收率为101.32%,RSD为0.94%(n=5)。本方法简便、快速、结果准确,适用于该制剂中盐酸小檗碱的质量分析检验。
An UV spectrophotometry method for the determination of berberine hydrochloride in ciprofloxacin hydrochloride and berberine hydrochloride premix was established with 0.05 mol/L sulfuric acid as reference solution at 421 nm. The calibration curve for berberine hydrochloride was linear within the concentration range of 8 - 56μg/mL(r =0.9992). The average recovery was 101.32%, and the RSD was 0.94% (n =5). This method was proved to be simple,precise, reproducible, and especially practicable for the quality analysis.
出处
《中国兽药杂志》
2007年第6期17-18,共2页
Chinese Journal of Veterinary Drug
关键词
盐酸环丙沙星
盐酸小檗碱预混剂
盐酸小檗碱
紫外分光光度法
含量
ciprofloxacin hydrochloride and berberine hydrochloride premix
berberine hydrochloride
UV spectrophotometry
content