葛根素对实验性糖尿病大鼠主动脉Ⅳ型胶原基因表达的抑制作用

Research on inhibitory effect of puerarin on aorta genetic expression of collage Ⅳ in diabetic rats

目的观察葛根素对实验性糖尿病大鼠主动脉Ⅳ型胶原基因表达的抑制作用。方法利用链脲佐菌素腹腔注射法诱导建立1型糖尿病大鼠模型,将实验用SD大鼠随机分为正常对照组、糖尿病组、葛根素3个剂量(20、40、80 mg.kg-1,ip)治疗组。治疗16周,观察治疗期间及治疗后大鼠的一般状况及血糖、糖化血红蛋白(HbA1c)的变化情况;用苏木精-伊红染色、原位杂交法检测主动脉内膜Ⅳ型胶原基因表达情况。结果造模4组大鼠均出现主动脉病理形态改变;葛根素能改善糖尿病模型大鼠的基本状况(体质量、精神状态等),降低糖化血红蛋白含量(P<0.05),降低主动脉Ⅳ型胶原基因(P<0.05)表达,且呈一定的剂量依赖关系。结论葛根素可通过抑制主动脉基因表达对糖尿病模型大鼠主动脉起到保护作用。

Objective To observe the inhibitory effect of puerarin on aorta genetic expression of collage Ⅳ in experimental diabetic rats. Methods Diabetic rats were induced by intraperitoneal injection of streptozotocin. Rats were allocated to normal control group, model group, and three puerarin groups （20, 40, 80 mg·kg^-1, i p）for 16 weeks. During and after the treatment, its general states（mind, physical quality）, blood sugar, and glycosylated hemoglobin（HbA1c） alteration of tissue morphology via hematoxylin and eosin staining were observed after the aorta was taken out from the rats executed by narcosis;mRNA of collage Ⅳ was determined by in situ hybridization analysis. Results Diabetes mellitus and aorta lesion occurred in the four model groups. Puerarin could improve the general state, and decrease glycosylated hemoglobin（P 〈 0.05）, the expression of collage Ⅳ mRNA in the aorta were significantly inhibited（P 〈 0.05）, and the action of puerarin was proved to be related to the dose. Conclusions Puerarin shows preventing effect on aorta by inhibiting aorta genetic expression of collage Ⅳ.