卡介菌多糖核酸对梅毒血清固定患者外周血T淋巴细胞亚群的影响

Effects of BCG-PSN on the T Lympocyte Subsets in Peripheral Blood of Patients with sero-resistance of Syphilis

目的研究卡介菌多糖核酸(BCG-PSN)对梅毒血清固定患者外周血T淋巴细胞亚群的影响,探讨BCG-PSN对梅毒血清固定可能的免疫调节机制。方法采用三色荧光抗体染色技术经流式细胞仪检测30例梅毒血清固定患者经卡介菌多糖核酸治疗前后外周血T淋巴细胞亚群,并与30例正常人群的检测结果相对照。结果BCG-PSN治疗前,梅毒血清固定患者外周血CD3+、CD4+淋巴细胞均低于正常对照组,差异有统计学意义(CD3+组P<0.001;CD4+组P<0.01),而CD8+淋巴细胞高于正常对照组,差异有统计学意义(P<0.001);经BCG-PSN治疗后,梅毒血清固定患者外周血CD3+、CD4+淋巴细胞均明显高于治疗前(P<0.001),CD8+淋巴细胞明显低于治疗前(P<0.001);治疗后梅毒血清固定患者外周血CD3+、CD4+、CD8+淋巴细胞与正常对照组差异无统计学意义(P>0.05)。结论卡介菌多糖核酸可通过调节梅毒血清固定患者外周血T淋巴细胞亚群,纠正CD4+/CD8+失衡,起到辅助治疗梅毒血清固定患者的作用。

Objective To study the effect of BCG-PSN on the Patients with Seroresistance of Syphili and to their immunity mechanisms. Methods Tlymphocyte subsets were measured by flow cytometry in peripheral blood of 30 sero-resistance and 30 healthy subjects. Results Before treatment using BCG-PSN, the proportion of CD3^＋ CD4^＋ T cell of peripheral blood from patients with Sero-resistance of Syphili were significantly decreased compared with those from NS （CD3^＋ group P〈0.001, CD4^＋ group P〈0.01）, the proportion of CD8^＋ T cell percentage was increased （P〈0.001）; After treatment using BCG-PSN, the proportion of CD3^＋ CD4^＋ T cell of peripheral blood from patients were significantly increased compared with those from NS （P〈0.001）, the proportion of CD8^＋ T cell percentage was significantly decreased compared with those from non-treated patients, （P 〈 0.001）; the proportion of CD3^＋ CD4^＋ T cell and the proportion of CD3^＋ CD8^＋ T cell from patients with Sero-resistance of Syphili had no significantly change in statistics （P〉0.05）, Conclusions BCG-PSN can regulate the T lymphocyte subset population and the ratio of CD4^＋ / CD8^＋ of peripheral blood from patients with Sero-resistance of Syphili. The Patients with Sero-resistance of Syphfli can be treated by BCG-PSN auxiliary field.