慢性乙肝患者外周血CD4^+细胞HLA-DR、CD8^+细胞CD28表达的检测及其意义

Detection of the expression of HLA-DR on CD4^+ T cells and CD28 on CD8^+ T cells on peripheral blood cells in patients with hepatitis B

目的检测乙肝患者外周血CD4+淋巴细胞表面HLA-DR和CD8+淋巴细胞表面CD28的表达情况,评价乙肝患者的细胞免疫状态。方法荧光抗体CD4-PECY5、HLA-DR-FITC和CD8-PE、CD28-FITC标记淋巴细胞,流式细胞仪分别测定患者CD4+淋巴细胞表面HLA-DR和CD8+淋巴细胞表面CD28表达的百分率,并与HBV-DNA结果比较。结果①与正常对照组比较,乙肝患者组外周血CD4+淋巴细胞表面HLA-DR的表达显著升高(P<0.001),CD8+淋巴细胞表面CD28的表达显著降低(P<0.01);②乙肝患者DNA阳性(>4000拷贝/ml)与阴性(<4000拷贝/ml)组CD4+淋巴细胞表面HLA-DR和CD8+淋巴细胞表面CD28的表达比较均无显著性差异(P>0.1)。结论乙肝患者外周血CD4+细胞的免疫活化增强,CD8+细胞与抗原递呈细胞结合的作用减弱;淋巴细胞的活化情况与病毒复制情况及含量多少无关。

Objective To study the expression of HLA-DR on CD4^＋ lymph cells and CD28 on CD8^ ＋ lymph cells on peripheral blood of hepatitis B evaluate patients＇ celluar immunity. Methods Lymph cells were marked with fluorescence antibody CD^4＋ PECYS, HLA-DR-FITC, CD8^＋, CD28. Then detected the percentage of the expression of HLA-DR on CD4^＋ cells and CD28 on CD8^＋ cells, and compared the results with HBV-DNA to study the relationship between celluar immunity and replication of virus. Results 0 Compared with the normal, the expression of HLA-DR on CD4^＋ cells in patients with B hepatitis was higher （P 〈 0.01）, the expression of CD28 on CD8^＋ cells was lower （ P 〈 0.01）. （1）There were no remarkable difference between in negative results of DNA （ 〈 4000 copy/ml） and in positive results of DNA （ 〉 4000 copy/ml）. Conclusion It suggested that the activation of CD^4＋ cells in hepatitis B was enhanced while the specific lethal effect of CD8^＋ cells was attenuated; the activation of lymph cells in patients with hepatitis B had no relationship with viral replication and the contents.