干扰素基因工程菌产物表达数学模型的研究

The Study of Mathematical Model for Product Formation with Recombinant Micrbes Expressed Interferon

根据阻遏蛋白，辅阻遏因子与启动基因之间的相互作用，建立了采用色氨酸启动子的基因工程重组微生物的产物表达数学模型。提出了ｍＲＮＡ最大转录速率常数和产物表达速率常数与比生长速率呈线性关系的假设，用干扰素基因工程菌培养过程中的实验数据估计了ｍＲＮＡ转录速率常数，干扰素表达速率常数和干扰素降解速率常数。推导出了发酵过程中干扰素表达量和细胞内干扰素比活计算公式。运用这些公式可以通过检测色氨酸浓度和细胞比生长速率来计算预测干扰素表达量。

Based on known molecular interactions of aporepressor,corepressor and inducer,a mathematical model of product formation about the Trp operon regulated recombinant microbes has been proposed,The linear relation of the overall transcription rate constant and overall translation rate constant on specific growth rate has been sugested.Used experimental data of which engineered microorganisms express interferon(IFN),Decay constant of IFN,mRNA transcription rate constant,IFN expression rate constant have been estimated.An equation ralated to IFN concentration in fermentation on specific growth rate and Trp concentration has been obtained.By application of the equation,the expression of IFN is calculated based on Trp concentration and specific growth rate in fermentation.Also,the optimum overall culture time can be determined.