真菌一氧化氮还原酶细胞色素P-450nor 2 cDNA序列的测定

Nucleotide Sequence of the Cytochrome P 450nor2 cDNA from Cylindrocarpon tonkinense

真菌一氧化氮还原酶细胞色素Ｐ４５０ｎｏｒ２ｃＤＮＡ序列的测定刘德立（华中师范大学生命科学学院武汉４３００７０）ＨｉｒｏｆｕｍｉＳＨＯＵＮ（筑波大学应用生物化学系日本）在真菌的反硝化作用中，一种细胞色素Ｐ４５０起着一氧化氮还原酶的作用，被称为细胞色...

The positive clones contained the Cytochrome P 450nor2 cDNA were screened with the antibody from the cDNA library of Cylindrocarpon tonkinense. The inserts were deleted by exonuclease Ⅲ,and sequenced by the dideoxy method.The full length sequence of the Cytochrome P 450nor2 cDNA was determined,which encoded a polypetide of 408 amino acid residues (Mr=45381).The N terminal region of amino acid sequence of the purified fungal C. P 450nor2 was consistent with that of the amino acid sequence reduced from P 450nor2 cDNA.