摘要
利用形态特征的观察、根尖细胞有丝分裂中期染色体计数、花粉母细胞减数分裂中期Ｉ（ＰＭＣＭⅠ）染色体构型分析以及Ｃ－分带，从普通小麦与大赖草（ＬｅｙｍｕｓｒａｃｅｍｏｓｕｓＬａｍ）杂种回交后代中选育出３个二体异附加系：９５Ｇ０４，９５Ｇ１６和９５Ｇ２３，其ＰＭＣＭⅠ染色体配对构型分别为０．１６Ⅰ＋２０．２５○Ⅱ＋１．６７Ⅱ，０．３５Ⅰ＋１８．９５○Ⅱ＋２．８７Ⅱ和０．１９Ⅰ＋２０．０７○Ⅱ＋１．８３Ⅱ。染色体Ｃ－分带结果表明：９５Ｇ０４，９５Ｇ１６，９５Ｇ２３分别为大赖草第１号，第４号。
By mitotic and meiotic analysis combined with plant morphology observation, scab resistance identification and chromosome C banding, three T. aestivum L. racemosus disomic addition lines (95G04, 95G16, 95G23) were isolated. Meiotic configurations at MⅠ in 95G04, 95G16 and 95G23 were 0 16Ⅰ+20.25○Ⅱ+1.67Ⅱ, 0.35Ⅰ +18.95○Ⅱ+2.87Ⅱ and 0.19Ⅰ+20.07○Ⅱ+1.83Ⅱ respectively. Metaphase chromosome C banding patterns indicated that Lr1, Lr4, Lr5 chromosomes were added to 95G04, 95G16, 95G23, respectively. The potential value of disomic addition lines and the transfer of superior traits were discussed.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1997年第2期6-11,共6页
Journal of Nanjing Agricultural University
基金
国家科委八五攻关项目
关键词
小麦
大赖草
异附加系
鉴定
Triticum aestivum L
Leymus racemosus Lam
alien disomic addition lines
identification
C banding
