螺旋藻富集同化无机硒研究

STUDY ON ASSIMILATION OF SPIRULINA TO INORGANIC SELENIUM

以亚硒酸钠做为螺旋藻富硒的来源，研究了亚硒酸钠梯度浓度对钝顶螺旋藻（Spirulinaplatensis）生长及其富硒量的影响，并采用^(75)Se标记的Na_2SeO_3示踪方法，对富硒螺旋藻含硒成分进行了研究。结果表明，当硒浓度超过360μg／mL时，螺旋藻的生长即受抑制，硒浓度为46μg／mL时，表现出促进螺旋藻生长的趋势，而硒浓度为184μg／mL时，螺旋藻可达到最高富硒量，平均为659.9ng／mg。富硒螺旋藻中结合在大分子上的有机硒达到70％以上，其中主要是含硒蛋白质，含量可达50％以上，主要以硒代蛋氨酸和硒代半胱氨酸形式存在，分别占含硒蛋白的40％和20％。

Sodiumm selenite, as a nutrient source of selenium was used to culture spirulina and the influence of gradient concentration of sodium selenite on the growth of Spirulina platensis and selenium enrichment content had been researched. Moreover,the selenized component of selenium-enriched spirulina was studied with the tracer technique of Se labeling Na_2SeO_3. The results showed that the growth of spirulina was inhibited as the selenium concentration beyond 360μ g/mL. Otherwise, trend of low selenium concentration (46μ g/mL) promoting the growth of spirulina was also showed.The highest selenium content of spirulina reached 659. 9ng/mg on an average. while the selenium concentration in medium was 184μ g/mL. In the selsnium-enriched spirulina,the content of organic selenium which were connected on the macromolecules amounted over 70%. the main selenized protein reached over 50%, seleno-methionine and selenocysteine accounted for 40% and 20% of selenized protein respectively.