期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

补肾阴对胚胎干细胞活动的影响 被引量：7

Effects of Tonifying Kidney-Yin on the Activities of Embryonic Stem Cell
下载PDF
导出
摘要 目的观察补肾阴中药复方对胚胎干细胞活动的影响。方法选用胚胎干细胞模型CRL-1825细胞株,加入补肾阴中药复方大鼠含药血清,观察补肾阴中药对干细胞分化、凋亡、细胞周期及其关键基因表达的影响。结果补肾阴中药复方含药血清可以抑制干细胞的分化和凋亡,促进干细胞增殖以及细胞周期的进程,促进干细胞Wnt、Oct4等基因的表达,下调P16INK4a基因的表达。结论补肾阴中药复方可以促进干细胞增殖、维持其不分化状态。 Objective To study the effects of tonifying kidney-yin on activities of embryo stem cell. Methods Embryonic stem cell line CRL- 1825 was choosed as model cell in present study, and tonifying kidney-yin herb serum was added to CRL-1825 cell. The influence of tonifying kidney-yin on stem cell differentiation, cell cycle, apotosis and express of key genes were observed. R, amltl Tonifying kidney-yin can inhibit stem cell differentiation and apotosis, promote stem cell proliferation and progression of cell cycle. In addition, tonifying kidney-yin can up-regulate Wnt, Oct4 gene expression, and down-regulation P16^INK4a expression. Conclusions Tonifying kidney-yin can promote stem cell proliferation and maintain the state of stem cell.
作者 安红梅 胡兵 史云峰 许丽雯 史秀峰 陈方敏 顾耘 潘露茜 王志
机构地区 上海中医药大学附属龙华医院 上海中医药大学
出处 《中国中医药信息杂志》 CAS CSCD 2007年第6期24-26,共3页 Chinese Journal of Information on Traditional Chinese Medicine
基金 国家自然基金资助(30400574)
关键词 补肾阴 中药 胚胎干细胞 Tonifying the kidney-yin Chiese herb embryo stem cell
分类号 R289.11 [医药卫生—方剂学]
  • 相关文献

参考文献1

二级参考文献53

  • 1[38]Butteroni C, FM De, H Scholer, M Pesce. Phage display screening reveals an association between germline-specific transcription factor Oct4 and multiple cellular proteins. J Mol Biol 2000; 304:52940.
  • 2[39]Scholer H, T Ciesiolka, P Gruss. A nexus between Oct4 and EIA: implications for gene regulation in embryonic stem cells. Cell 1991; 66:291-304.
  • 3[40]Curatola AM, C Basilico. Expression of the K-fgf proto oncogene is controlled by 3' regulatory elemnents which are specific for embryonal carcinoma cells. Mol Cell Biol 1990; 10:2475-84.
  • 4[41]Yuan H, N Corbi, C Basilico, L Dailey. Developmentalspecific activity of the FGF4 enhancer requires the synergistic action of Sox-2 and Oct-3. Genes Dev 1995;9:263545.
  • 5[42]Ambrosetti DC, C Basilico, L Dailey. Synergistic activa tion of the fibroblast growth factor 4 enhancer by Sox2 and Oct-3 depends on protein-protein interactions facilitated by a specific spatial arrangement of factor binding sites. Mol Cell Biol 1997; 17:6321-9.
  • 6[43]Ambrosetti DC, HR Scholer, L Dailey, C Basilico. Modu lation of the activity of multiple transcriptional activation domains by the DNA binding domains mediates the synergistic action of Sox-2 and Oct-3 on the fibroblast growth factor4 enhancer. J Biol Chem 2000; 275:23387-97.
  • 7[44]Tomioka M, M Nishimoto, S Miyagi, T Katayanagi, N Fukui, H Niwa, M Muramatsu, A Okuda. Identification of Sox-2 regulatory region which is under the control of Oct-3/4-Sox-2 complex. Nucleic Acids Res 2002; 30:3202- 13.
  • 8[45]Nishimoto M, A Fukushima, A Okuda, M Muramatsu. The gene for the embryonic stem cell coactivator UTF1 carries a regulatory element which selectively interacts with a complex composed of Oct-3/4 and Sox-2. Mol Cell Biol 1999; 19:5453-65.
  • 9[46]Wakayama T, R Yanagimachi. Cloning the laboratory mouse. Semin Cell Dev Biol 1999; 10:253-8.
  • 10[47]Wakayama T, H Tateno, P Mombaerts, R Yanagimachi. Nuclear transfer into mouse zygotes. Nat Genet 2000; 24:108-9.

共引文献37

同被引文献113

引证文献7

二级引证文献46

中国中医药信息杂志
2007年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部