蓬甲饮治疗子宫内膜异位症SD大鼠的作用机制探讨

Mechanism of Pengjiayin Preparation in the Treatment of Ectopic Endometrium in SD Rats

目的探讨蓬甲饮治疗子宫内膜异位症的作用机理。方法建立子宫内膜异位症大鼠模型,应用免疫组化法检测凋亡调节蛋白Caspase-3、Survivin在诱发性子宫内膜异位症动物模型的异位、在位内膜中的表达,从免疫的角度研究蓬甲饮、乌丹丸治疗子宫内膜异位症的作用机制。同时,用TUNEL法检测出细胞的凋亡率。结果与空白组比较,蓬甲饮低、中、高剂量组及乌丹丸组Caspase-3的表达增加(P<0.05),与空白组比较,蓬甲饮中、高剂量组及乌丹丸组Survivin的表达减少(P<0.05)。结论蓬甲饮和乌丹丸均增加Caspase-3的表达,降低Survivin的表达,促进细胞凋亡。

Objective To explore the mechanism of Pengjiayin preparation in the treatment of ectopic endometrium in SD rats. Methods To study the mechanism of Wudan Pill and Pengjiayin preparation in the treatment of ectopic endometrium in immunological way. SD rat models of endometriosis were established, and the expression of Survivin and Caspase-3 protein associated with apoptosis was detected in ectopic and eutopic endometrium in rats with the immunohistochemical method, simultaneously apoptotic cells were detected by TUNEL. Results Compared with the control group, the expression of the caspase-3 was increased in the low dosage group, the midium dosage group and the high dosage group of the Pengjiayin preparation, and the increase was also observed with the Wudan Pill group （P〈0.05）. While comparing with the control group, the expression of survivin was decreased in the midium dosage group and the high dosage group of the Pengjiayin preparation, and also in the Wudan Pill group （P〈0.05）. Conclusion Pengjiayin preparation and Wudan Pill both can increase the expression of the caspase-3 and decreased the expression of the survivin, which promoting the apoptosis of ectopic endometrium.