痛清颗粒中芍药苷与葛根素的提取工艺研究

Optimization of the Technology of Extracting the Paeoniflorin and Puerarin from Tongqing Granules

目的优选痛清颗粒中芍药苷与葛根素的提取工艺。方法采用4因素3水平的正交试验设计法,以芍药苷、葛根素转移率综合评分为考核指标,筛选影响提取工艺的因素。结果实验设计三因素没有显著性影响。结合生产实际需要,痛清颗粒中芍药苷与葛根素的最佳提取条件是:加入22倍量水提取3次(12,5,5),提取2h(1.0,0.5,0.5)。结论此条件为痛清颗粒中芍药苷和葛根素的最佳提取工艺。

Objective To optimize the technology of extracting Paeoniflorin and Puerarin from Tongqing Granules. Methods The experiment used the orthogonal experimental design （4 factors and 3 levels） with the shift-rate of Paeoniflorin and Puerain as experimental criterion in screening the factors affecting the technology of extracting Tongqing Granules. Results All the 3 factors designed in the orthogonal experiment non-signifantly affected the extracting technology. In consideration of the industrial practice, the best condition of extracting the constituents from Tongqing Granules was decided： water extraction for 3 times, extracting with 12 fold water for i hours for the first time, with 5 fold water for 0.5 hours for the second time, and with 5 fold water for 0.5 hour for the third time. Conclusion The condition is the optimized technology of extracting Paeoniflorin and Puerarin from Tongqing Granules.