黑荆树树皮原花色素生物活性的研究(英文)

Investigation on Biological Activities of Proanthocyanidins from Black Wattle Bark

对黑荆树树皮中原花色素的提取及生物活性进行了研究。实验分别以水、甲醇和乙醇作为提取溶剂,对黑荆树皮中原花色素的提取进行了探讨,通过测定其总抽出物含量和原花色素粗提物得率的影响,确定了适宜的提取条件,并重点对原花色素生理活性包括抗肿瘤和抗氧化活性进行了评价。利用MTT比色法对原花色素抗肿瘤活性进行了测定,利用清除DPPH自由基能力测定了其抗氧化能力。实验结果表明,黑荆树树皮是一种富含原花色素的植物资源。提取溶剂选择性试验结果表明,乙醇作为提取溶剂,不仅可以得到较高提取率的原花色素,而且所得原花色素粗提物具有较高的生理活性。黑荆树树皮原花色素适宜提取工艺条件为:溶剂浓度70%,提取温度60℃,液料比7∶1。体外癌细胞培养的抑制作用测试结果显示,黑荆树树皮提取得到的原花色素粗产品对人早幼粒白血病细胞HL-60有中效,分别对人低分化胃腺癌细胞BGC-823和人肝癌细胞BEL-7402有弱效。自由基清除能力测定结果显示黑荆树树皮中原花色素提取物有很强的抗氧化活性。

The extraction conditions of proanthocyanidins from bark of black wattle （Acacia mearnsii De Wilde） and their biological activities were investigated in this study. Different types of solvents such as water, methanol and ethanol were used as extraction solvent, respectively. Experiments were conducted to study the effects of temperature, liquid-solid ratio and concentration of ethanol on the extraction efficiency.The focus of this study is to evaluate the biological activities of the extracted proanthocyanidins products. The anti-tumor activity of proanthocyanidins was determined by MTT colorimetric method, and DPPH method referred to the radical scavenging capacity was used to test the anti-oxidation ability of the proanthocyanidins. The results showed that black wattle bark is rich in proanthocyanidins. Ethanol was considered to be a suitable solvent, in which there was higher extract yield and stronger biological activities of proanthocyanidins. It was found that the optimum conditions of extraction were as follows ： concentration of ethanol 70 %, temperature 60 ℃ ,and the ratio of solution to solid 7 ： 1. Furthermore, the results of anti-tumor test of proanthocyanidins crude products on human cancer cells cultured in vitro showed that the proanthocyanidins had medium effect on promyelocytic cell line HL-60, weak effect on human stomach adenocarcinoma BGC-823 and human hepatocellular carcianoma BEL-7402. Meanwhile, the results of radical scavenging ability assay indicated that the proanthocyanidins had strong anti-oxidation activity.