HIV抗体ELISA阳性标本蛋白印迹确证试验的研究

WESTERN BLOT ASSAY RESEARCH TO THE HIV ANTIBODY ELISA POSITIVE SPECIMENS

用获ＦＤＡ准许的美国ＣａｍｂｒｉｄｇｅＢｉｏｔｅｃｈ公司ＨＩＶ－１蛋白印迹试剂盒（ＷＢ）对ＨＩＶ－１／２抗体诊断试剂盒（ＥＬＩＳＡ）初筛阳性的６２份血清标本做确证试验。一次性确证ＨＩＶ抗体阳性者８名；阴性者１２名；不确定者４２名。对８名ＨＩＶ抗体不确定者做追访采样监测，其中１人１２天后ＷＢ血清ＨＩＶ抗体由不确定性转为阳性，另７人在６～１５个月经２～３次采样检测，ＷＢ区带反应无变化或略有变化。本研究证实ＷＢ不确定者，若有ＨＩＶｅｎｖ（ｇｐ１６０、ｇｐ１２０、ｇｐ４１）区带反应，ＨＩＶ血清抗体可能转阳；若仅ＨＩＶｐｏｌ（ｐ３１、ｐ５１、ｐ６６）和／或ｇａｇ（Ｐ１７、Ｐ２４、Ｐ５５）区带反应，很大可能是非特异性反应。虽ＥＬＩＳＡ阳性，而ＷＢ不确定者血清主要呈ｐ２４抗体反应（４２．９％，１８／４２）与ｐ２４、ｐ１７抗体反应（２８．６％，１２／４２）。

Total 62 HIV-1/2 ELISA screening positive samples were confirmed with US FDA licensed Cambridge Brotech HIV-1 Western Blot Kit,8 positive,12 negative and 42 indeterminate test resultes were confirmed one time.Follow-up testing 8 HIV WB-indeterminate specimens,one individual of them turned into WB-positive after 12 days,the other 7 collected specimens were tested 2-3 times by WB within 6～15 months,their WB reactivity bands were no change or a bit.Our experience indicates that WB-inditerminate serum HIV-antibody would change into WB-positive if there is HIV Eev (gp160 or gp120 or gp41)band on a strip.When there are just only HIV Pol(p31,p51,p66)and /or gag (p17,p24,p55)reactivity bands it will be properly nonspecific reaction.The most common band patterns of WB-indeterminate specimens are p24 (42.9%,18/42)and p24.p17(28.6%,12/42).