高效液相色谱法测定退障凝胶中大黄素、大黄酚的含量

Quantitative Determination of Emodin and Chrysophanol in Tuizhang Hydrogel by HPLC

目的:建立高效液相色谱法测定退障凝胶中大黄素、大黄酚含量的方法。方法:采用Waters高效液相色谱仪(Waters 2695-2487系统);Empower色谱工作站;色谱柱Kromasil C18(4.6 mm×250 mm,5μm);流动相为甲醇-0.1%磷酸溶液(82∶18);紫外检测波长254 nm;流速1.0 mL.min-1;进样量20μL。结果:大黄素在0.02～0.125μg范围内呈现良好的线性关系,大黄酚在0.072～0.360μg范围内呈现良好的线性关系,大黄素、大黄酚的加样回收率分别为99.1%,99.2%。结论:该法简便,准确,具有专属性,可用于退障凝胶的质量控制。

Objective： To develop an HPLC method for analysis of emodin and chrysophanol in Tuizhang Hydrogel. Method： The emodin and chrysophanol in Tuizhang Hydrogel were separated on a Chromasil C18 column （4.6 mm × 250 mm,5 μm） eluted with the mobile phase of methanol-0.1% Phosphoric acid （82： 18）. The flow-rate was 1.0 mL· min^-1, and the UV detector wavelenghth was set at 254 nm. Results：The calibration curves were linear over the concentration range of 0. 025 - 0.125 μg for emodin and 0. 072 - 0. 360 μg for chrysophanol. The recoveries of the emodin and chrysophanol were 99.1%, 99.2 %, respectively. Conclusion： The method was simple and accurate, it can be used for the determination of emodin and chrysophanol in Tuizhang Hydrogel.