摘要
目的观察蛋白激酶C(protein kinase C,PKC)同工酶在乳腺癌组织中的表达状况,探讨癌细胞信息传递的分子生物学机制。方法应用半定量RT-PCR检测了31例乳腺癌组织与12例癌旁正常乳腺组织中PKC-βΙmR-NA表达;应用Western blot检测以上组织中PKC-βΙ蛋白表达。结果乳腺癌组织中PKC-βΙmRNA与蛋白表达均高于癌旁正常乳腺组织(P<0.001)。结论乳腺癌组织中存在PKC-βΙ同工酶过度表达,它可能介导乳腺癌细胞分化的信号转导过程。
Objective Expression of protein kinase C（PKC） iscenzymes in breast cancer tissues was studied to explain the intercellular signal transduction molecular mechanisms. Methods Expression of PKC- β1 was assayed by semiquantitative RT- PCR end Western blotting in 31 patients＇ breast cancerous tissues end 12 samples of normal breast tissues. Re- sult PKC - β1 mRNA end protein were significantly increased in cancerous tissues as compared with the normal breast tissues （P〈 0. 001）. Conclusion Overexpression of PKC-β1 plays an importent rote in regulating cellular proliferation of breast cancer.
出处
《实用预防医学》
CAS
2007年第3期628-630,共3页
Practical Preventive Medicine
基金
湖南省教育厅基金项目(批准号06C786)
