高胆固醇血症大鼠自由基代谢紊乱及阿托伐他汀的干预作用

Free radical metabolic disorder and intervention of atorvastatin on hypercholesteremia rats

目的:研究阿托伐他汀对高胆固醇血症大鼠低密度脂蛋白氧化修饰的干预作用。方法:采用脂肪乳剂灌胃的方法建立高胆固醇血症大鼠模型,30只wistar大鼠随机分为对照组、模型组和阿托伐他汀(ATV)组(5mg/kg.d),每组10只。给药4周后,采用酶法、ELISA等方法测定血清总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-c)、高密度脂蛋白胆固醇(HDL-c)、超氧化物歧化酶(SOD)、丙二醛(MDA)、氧化低密度脂蛋白(ox-LDL),并计算(TC-HDL-c)/HDL-c和LDL-c/HDL-c。结果:模型组TC、LDL-c、(TC-HDL-c)/HDL-c、LDL-c/HDL-c、ox-LDL、MDA显著高于对照组,HDL-c、SOD显著低于对照组(P<0.01);同模型组比较,阿托伐他汀能够显著降低TC、LDL-c、ox-LDL、MDA,升高HDL-c、SOD(P<0.01)。结论:阿托伐他汀能够降低循环系统氧化低密度脂蛋白水平,具有较好抗脂质过氧化的作用,可能是其抗动脉粥样硬化机制之一。

Objective：To study the LDL oxidation in hypercholesteremia rats and the effects of atorvastatin. Methods：The experimental model of hypercholesteremia was established in rats by ig. fat emulsion. 30 wistar rats were sub-divided randomly into the control group ,the model group and the atorvastatin group （ 5 mg/kg · d）. After 4 weeks, the serum TC, LDL-c, HDL-c, SOD, MDA, ox- LDL were detected by enzyme or ELISA methods. In addition, （TC-HDL-c）/HDL-c and LDL-c/HDL-c were calculated. Results： Contrast with control group, LDL, LDL-c, （ TC-HDL-c ）/HDL-c, LDL-c/HDL-c, ox-LDL, MDA were markedly higher, while HDL-c and SOD were markedly lower in model group （ P 〈 0. 01 ）. LDL, LDL-c, （TC-HDL-c）/HDL-c, LDL-c/HDL-c, ox-LDL, MDA were markedly lower in ATV group than that in model group（ P 〈 0. 01 ） ; HDL-c and SOD were significantly higher in ATV group than that in model group（ P 〈 0. 01 ）. Conclusion：Atorvastatin decreased blood lipid, inhibited the lipid peroxidation and the oxidative modification of LDL of rat serum. This result may be one of mechanisms in preventing atherosclerosis.