MCM5和Ki67在宫颈上皮内瘤变及宫颈癌组织中表达及意义

Expressions of MCM5 and Ki67 in cervical intraepithelial neoplasia and cervical cancer and their clinical significance

目的探讨微小染色体维持蛋白-5（minichromosome maintenance-5,MCM5）和Ki67在宫颈上皮内瘤变（cer-vical intraepithelial neoplasia,CIN）及宫颈癌组织中的表达及临床病理意义。方法采用免疫组化技术（S-P法）检测MCM5和Ki67在99例宫颈CIN和宫颈癌中的表达。结果MCM5和Ki67在正常宫颈黏膜上皮、CINⅠ-Ⅱ级、CINⅢ级、浸润癌表达的阳性率分别为26.7%、72.0%、80.0%、97.9%和10.0%、32.4%、84.0%、97.9%。各级CIN与浸润癌相比MCM5的表达率差异具有显著性（P〈0.05）;在CINⅢ级和宫颈癌中MCM5的表达与Ki67表达呈正相关（r=0.687,0.936,P〈0.05）。MCM5和Ki67表达率与临床分期及病理分级有关（P〈0.05）。结论MCM5和Ki67可作为反映宫颈上皮增生病变程度及生物学行为的指标,对宫颈非浸润性病变与浸润性病变的诊断及鉴别有参考价值。

Objective To study the expressions of MCM5 and Ki67 in cervical intraepithelial neoplasia （CIN） and cervical cancer and their clinical significance. Methods Immunohistochemistry （S-P method） was used to detect the expressions of MCM5 and Ki67 in 50 CIN and 49 cervical cancer. Results The positive expression rate of MCM5 and Ki67 in normal cervical epithelium, CIN Ⅰ - Ⅱ , CIN Ⅲand cervical cancer were 26.7%, 72.0%, 80.0%, 97.9% and 10.0%, 32.4%, 84.0%, 97.9% respectively. The positive expression rate of MCM5 was significantly different between CIN and cervical cancer （ P 〈 0. 05 ）. The expressions of MCM5 and Ki67 had a positive correlation in CIN Ⅲ and cervical cancer （P 〈 0. 05）. The positive expressions of MCM5 and Ki67 were closely correlated with clinical stage and pathological grade of cervical cancer （P 〈 0. 05）. Conclusion MCM5 and Ki67 may be used as markers for aggressive biological behavior in cervical cancer, and may be useful to distinguish CIN and cervical cancer.