S-甲基异硫脲治疗颞下颌关节骨关节病的动物实验研究

Animal Experimental Study of Intra-articular Injection of S-methylisothiourea for Treatment of Temporo- mandibular Joint Osteoarthrosis

目的探讨S-甲基异硫脲(SMT)对黑山羊颞下颌关节骨关节病的治疗效果。方法选取9只纯种黑山羊,随机分为正常对照组、实验对照组和实验组,每组3只。对实验组和实验对照组黑山羊双侧颞下颌关节上腔一次性注射胶原酶,诱导骨关节病病变。正常对照组不作任何治疗;实验对照组为对实验动物关节上腔注射生理盐水0.5mL;实验组为对实验动物关节上腔注射SMT0.5mL。处死动物后在光镜和扫描电镜下观察颞下颌关节的变化。结果在光镜和扫描电镜下观察,实验对照组髁突、关节盘、关节凹表现为骨关节病变化;实验组中则表现出不同程度的改善。结论通过3个月的观察和研究,发现SMT关节上腔重复注射能抑制颞下颌关节骨关节病的发展,其作用机制可能与SMT抑制一氧化氮合酶在关节局部产生过多的一氧化氮有关。

Objective To investigate the efficacy of S-methylisothiourea（SMT） for treatment of temperomandibular joint osteoarthrosis（TMJOA） of goats. Methods Nine purebred black goats were randomly devided into three groups： Normal control group, control group and experimental group. The upper compartments of both temporomandibular joint of the goats in control group and experimental group were injected with collagenase only once to induce osteoarthrosis. Normal control group received no treatment. The upper compartments of both TMJ were injected with 0.5 mL of normal saline, and experimental group, the upper compartments of both TMJ were injected with 0.5 mL of SMT. The TMJ of goats was examined with scanning electron microscopy and microscopy after sacrificed. Results Examined in light microscopy and scanning electron microscopy, normal control group showed normal performance, the control group showed severe osteoarthrotic changes in the temporal surface, disk and condyle, while the experimental group showed improvement of different degree. Conclusion On the basis of 3-month following-up study, repeated intra-articular injection of SMT may play a role in inhibiting TMJOA progression.