颞下颌关节骨关节病发展过程中诱导型一氧化氮合酶在其软骨组织内的表达和意义

Expression of Inducible Nitric Oxide Synthase in Cartilage in Progression of Temporomandibular Joint Osteoarthritis

目的研究颞下颌关节骨关节病(TMJOA)发生发展过程中诱导型一氧化氮合酶(iNOS)在其软骨组织内的表达和作用。方法采用山羊建立TMJOA动物模型,在各组动物注射胶原酶后的2、4、12、24周时,分别切取其TMJ标本做EnVision二步法免疫组化检测。结果正常组TMJ软骨无iNOS表达,在实验组动物TMJOA发病过程中其软骨组织iNOS的表达有明显增加,且随着病变过程的发展与病变组织有一定的对应性。结论iNOS在TMJOA发生发展过程中起着重要的作用,抑制iNOS的生成有可能会阻断TMJOA的发生和发展。

Objective To study the expression of inducible nitric oxide synthase （iNOS） in cartilage of temperomandibular joint osteoarthriti （TMJOA）, and to evaluate the role of iNOS in the progression of TMJOA. Methods The goats were used and TMJOA was induced by injection of collagenase in upper joint space. The joints were obtained and were investigated by using immunohistochemistry at 2, 4, 12, 24 weeks after injection. Results Almost no expression of iNOS in normal cartilage of TMJ. In the diseased joints, strong or definite iNOS reactivity was expressed. Conclusion iNOS plays an important role in the progression of TMJOA.