桂皮醛对大鼠根尖周炎根管中内毒素水平影响的定量研究

Quantitative Study of Effects of Cinnamaldehyde on Levels of Endotoxin in Root Canals in Rats′ Periapical Periodontitis Model

目的探讨桂皮醛对大鼠根尖周炎根管中内毒素水平的影响,为桂皮醛作为根管消毒药提供实验依据。方法用75只Wistar大鼠建立根尖周炎模型,随机分为3组,分别为桂皮醛组、甲醛甲酚组、生理盐水组,每组25只。封药前后分别用显色基质鲎试剂法测内毒素的含量。结果根管中内毒素含量结果显示,桂皮醛和甲醛甲酚组明显下降(P<0.05),但两组差异无统计学意义(P>0.05),生理盐水组无明显变化(P>0.05)。结论桂皮醛能有效地降低感染根管中内毒素的含量。

Objective To provide experimental evidence for the exploitation of cinnamaldehyde as a kind of root canal disinfectant through studying the effect of cinnamaldehyde on endotoxin in root canals. Methods This experimental model of periapical periodontitis was established with Wistar rats. The 75 rats were divided randomly into 3 groups： Group of cinnamaldehyde, group of formaldehyde cresol formocresol, group of physiological saline. The level of endotoxin was measured by quantitative chromogenic tachypleus amebocyte lysate method before and after sealing the drugs in the root canal. Results The level of endotoxin in the group of cinnamaldehyde and formaldehyde cresol formocresol decreased obviously （P〈0.05）, and the difference between them was of no significance （P〉0.05）, the group of physiological saline was of no significant difference （P〉0.05）. Conclusion Cinnamaldehyde can decrease the level of endotoxin obviously.