流式细胞术检测成人急性髓细胞白血病P糖蛋白表达的临床意义

Clinical Significance of Flow Cytometric Determination of P-glycoprotein Expression in Adult Acute Myeloid Leukemia

应用P糖蛋白（Pgp）单抗UIC2及流式细胞术间接免疫荧光法检测75例初治成人急性髓细胞白血病（AML）Pgp表达，并探讨其临床意义。结果23例（30．7％）表达Pgp，19例（82．6％）Pgp+AML共表达CD34．Pgp表达与初诊性别、年龄、肝脾肿大、外周血象等无明显相关性．M3中未见Pgp表达。伴t（8；21）及t（15；17）染色体异常的AMLPgp表达低。Pgp+及Pgp－AML之完全缓解率分别为26．1％及76．9％（P＜0．05）．CD34+与CD34－AML的CR率分别为42．9％及84．8％（P＜0．05），Pgp+／CD34+与Pgp-／CD34－AML的CR率分别为15．8％及86．2％（P＜0.05）。结果表明Pgp及CD34是成人AML预后差的重要原因。

The P-glycoprotein(Pgp) in 75 Patients suffering from untreated adult acute myeloid leukemia(AML) previously was assayed by monoclonal antibody U1C2 with FCM. The Pgp was positive(≥10% ) in 30. 7% of cases and coexpressed with CD34 antigen in the most cases of Pgp+AML. There was no relationship between Pgp expression and sex, age,heptospienomegaly,peripheral bloodgarms or FAB subsets except for M3 subtype which had no Pgp expression. The complete remission(CR) rate of Pgp+AML(26. 1 % ) was significantly lower than that of Pgp-AML(76. 9%). Furthermore,CR rate of Pgp+/CD34+AML and Pgp-/CD34-AML was 15. 8% and 86. 2% respectively. These suggest that Pgp is an index of poor prognosis for patients suffering from untreated adult AML previously.