E-选择素基因G98T多态性对原发性高血压患者血压及心脏结构功能的影响

Effect of E-selectin G98T polymorphism on blood pressure,cardiac structure and cardiac function in patients with essential hypertension

目的 研究年龄在E-选择素G98T基因多态性对高血压影响中的作用。方法 高血压组326例，对照组216例。对两组进行血压测量，检测生化指标及NO、ET、AI、AII、ALD。以PCR-RFLP方法进行DNA多态性分析，电泳判断基因型并测序。结果 检测到TT、GG、GT三种基因型。在〈40岁组，携带T等位基因发生高血压的危险性为G的6.294倍（P=0.007;OR=6.294;95%CI：1.361-29.11）。在40-60岁及〉60岁组，高血压组与对照组的等位基因频率则无显著差异（P=0.528和0.965）。〈40岁的HT组中TT-TG基因型CSBP、CDBP及MAP显著高于GG型（P分别为0.006、0.023和0.036）。在40-60岁组：TT-TG基因型的CSBP、MAP也显著高于GG基因型（P分别为0.031和0.042），而〉60岁组，两基因型间血压无显著差异。在〈40岁的HT组中TT-TG基因型LVMI显著大于GG型（P=0.042）。结论 E-选择素G98T基因多态性对高血压有显著影响，T等位基因携带者发生高血压风险大，血压值较高，更倾向于发生左室肥厚。这种影响随着年龄的增长而减弱。

Objective To investigate how age affect the effect of E-selectin G98T polymorphism on blood pressure. Methods Genotypes of 326 essential hypertension patients （male 173, female 153, age [47.12±13.22] y, BMI [26.16±3.33] kg/m^2） and 216 healthy individuals （male 60,female 156, age [40.04±11.38] y, BMI [24.82±3.04] kg/m^2） were typed by polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP）. Took blood pressure and examed BG, TG, TC, LDL-c, HDL-c, UA, BUN, NO, ET, AI, All, ALD of each individuals. Results Three genotypes （TT, GG, GT） were found. In 〈40 y group, risk of hypertension for allele T is 6. 294 times of that for allele G （P=0. 007; OR= 6. 294; 95%CI; 1.361-29.11）. While in 40-60 yand 〉60 ygroup, there were no significantly different ingenotype between HT group and N group. In 〈40 y HT group, the CSBP, CDBP and MAP of TT-TG genotype are higher than that of GG （P=0. 006, 0. 023 and 0. 036）. In the same group, the LVMI of TT-TG is higher than that of GG （P=0. 042）. Conclusion E-selectin G98T polymorphism affect blood pressure significantly. T allele has higher risk for hypertension and left ventricular hypertrophy. But this effect are affected by age.