抗胆碱能药物分子烙印聚合物的分子识别研究

Study on Molecular Recognition of Molecularly Imprinted Polymers with Anticholinergic Drugs as Templates

目的探讨分子烙印聚合物(molecularly imprinted polymer,MIP)分子识别能力的机制及影响因素。方法以抗胆碱能药物盐酸新托品为模板分子、甲基丙烯酸为功能单体、三羟甲基丙烷三甲基丙烯酸酯为交联剂在乙腈中合成MIP,采用固相萃取-高效液相色谱方法考察MIP对乙腈溶液中的模板分子和结构类似物盐酸苯环壬酯、盐酸戊乙奎醚及结构有一定差异的化合物樟柳碱和氯苯那敏的固相吸附行为。结果MIP的分子识别能力与其合成条件和实验条件密切相关。结论MIP对待测物的非特异性吸附主要由氢键作用引起;MIP的特异性识别过程中,识别位点与待测物的空间结构匹配起着更为重要的作用。

OBJECTIVE To investigate the molecular recognition mechanism of molecularly imprinted polymer （MIP） and its influence factors, METHODS MIPs were prepared with anticholinergic drug neotropine as template, methacrylic acid （MAA） as functional monomer and trimethylolpropane trimethacrylate （TRIM） as crosslinker. The specific solid phase extraction of MIPs was investigated for anticholinergic drugs ： neotropine, penequinine, bencynonatine and anisodine, chlorphenamine with different hydrogen-band groups. RESULTS The results showed that the recognition ability of MIPs was associated closely with the conditions of synthesis and experiment. The specific molecular recognition was observed for anticholinergic drugs and weak adsorption for anisodine and chlorphenamine. CONCLUSION The experiments reveal that the non-specific adsorption of MIPs could mainly result from the hydrogen-band of polar groups between MIPs and analytes. The steric shape complementary between analytes and the recognition sites of MIPs plays a key role in the molecular recognition progress.