EAE大鼠外周免疫器官细胞凋亡的研究

A study of cell apoptosis in the peripheral lymphoid organs in EAE rats

目的:研究实验性变态反应性脑脊髓炎(EAE)大鼠外周免疫器官中细胞凋亡的情况。方法:应用脱氧核糖核苷酸末端转移酶介导的缺口末端标记(TUNEL)技术和流式细胞仪定量分析动态检测EAE病程不同阶段大鼠外周免疫器官中细胞凋亡的变化情况。结果:实验组脾和淋巴结中细胞凋亡率很低,组间及组内均无显著差异。结论:外周免疫器官在EAE凋亡中不起主要作用。

Objective： To investigate the apoptotic cell in the peripheral lymphoid organs in experimental autoimmune encephalomyelitis（EAE） rats. Methods： The status of apoptofic cells were detected in the peripheral lymphoid organs in EAE in various stages of the course, performing TdT-mediated-dUTP nick end labeling（TUNEL） and flow cytometric analysis（FCM） of their DNA stained with propidium iodide. Results： The rate of cell apoptosis by FCM and the number of TUNEL-positive cells were very low in the lymph nodes and spleens, while no significant difference was found either within or between the two groups. Conclusions： Apoptosis in the peripheral lymphoid organs plays an unimportant role in EAE.