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趋化因子MCP-1、MIP-1α在EAE小鼠脊髓中的表达 被引量：8

The expressions of MCP-1 and MIP-1αin spinal cord of EAE mice
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摘要 目的:研究单核细胞趋化蛋白(MCP)-1和巨噬细胞炎性蛋白(MIP)-1α与实验性自身免疫性脑脊髓炎(EAE)发病的关系。方法:用髓鞘少突胶质细胞糖蛋白_(35-55)多肽加福氏完全佐剂皮下注射免疫C57BL/6小鼠建立EAE模型,用免疫组织化学方法观察EAE小鼠发病后第0天(初期)、第7天(高峰期)及第30天(恢复期)脊髓中MCP-1、MIP-1α的表达的变化,并通过免疫组化染色标记星形胶质细胞及小胶质细胞,判断MCP-1、MIP-1α的细胞来源。结果: MCP-1、MIP-1α在EAE小鼠发病初期脊髓中有少量表达,发病高峰期表达增高,而恢复期无表达,MCP-1主要由星形胶质细胞产生,MIP-1α主要由小胶质细胞产生;对照组小鼠脊髓中则没有MCP-1、MIP-1α表达。结论:趋化因子MCP-1、MIP-1α在EAE小鼠CNS不同胶质细胞中表达,是EAE发病早期募集免疫反应细胞向CNS浸润的重要致炎性因子。 Objective： To investigate the role of monocyte chemoattractant protein- 1 （MCP- 1） and macrophage inflammatory protein- 1α （MIP- 1α） in the pathogenesis of experimental autoimmune encephalomyelitis （EAE）. Methods： EAE models in C57BL/6 mice were induced by injecting subcutaneously myelin oligodendrocyte glycoprotein （35-55） peptide in complete Freund＇s adjuvant. The expressions of MCP-1 and MIP-1α in the spinal cord at different stages of EAE were observed and the cellular source of MCP-1 or MIP-1α in CNS were identified respectively by staining using cell phenotype markers. Results： Reactive hypertrophic astrocytes were strongly immunoreactive for MCP-1, while MIP-1α was produced mainly by microglia. The productions in the CNS of these two chemokines were increased to correlate with disease development. Conclusion： Chemokines MCP-1 and MIP-1α are expressed in different glial cells in the CNS of EAE mice and are important proinflammatory cytokines for trafficing immune cells to infiltrate into CNS at initial stage of EAE.
作者 徐全刚 王晓辉 田书娟 任晓暾 吴卫平
机构地区 解放军总医院神经内科 解放军总医院基础所生物化学研究所
出处 《脑与神经疾病杂志》 2007年第3期216-218,209,共4页 Journal of Brain and Nervous Diseases
关键词 实验性自身免疫性脑脊髓炎 多发性硬化 趋化因子 单核细胞趋化蛋白 巨噬细胞炎性蛋白 Experimental autoimmune encephalomyelitis Multiple sclerosis Chemokine Monocyte chemoattracant protein Macrophage inflammatory protein
分类号 R744.51 [医药卫生—神经病学与精神病学]
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参考文献8

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二级参考文献13

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脑与神经疾病杂志
2007年 第3期

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