CO_2气腹对人宫颈癌CaSki细胞Survivin和Bcl-2表达的影响

Effect of imitated CO_2 pneumoperitoneum on expressions of Survivin and Bcl-2 in human cervical carcinoma cell line

目的探讨CO2气腹对人宫颈癌CaSki细胞抗凋亡能力的影响。方法建立腹腔镜CO2气腹体外模型,以不同压力(0、7、14mmHg)的CO2气腹对人宫颈癌CaSki细胞处理不同时间(1、2、3、4h),设未处理为对照组。采用RT-PCR、Western blot分别检测CO2气腹处理后各组细胞Survivin mRNA和Bcl-2蛋白的表达情况。结果当压力为7、14mmHg时,Survivin和Bcl-2的表达均随着气腹处理时间的延长呈上升趋势(P<0·05)。当处理相同时间时,7mmHg组Survivin和Bcl-2的表达量均明显高于其他压力组和对照组(P<0·05)。结论在一定压力和作用时间范围,CO2气腹能提高宫颈癌细胞的抗凋亡能力,这可能是CO2气腹促肿瘤生长的机制之一。

Objective To study the effect of CO2 pneumoperitoneum on the anti-apoptotic capability of human cervical carcinoma cell line （CaSki）. Methods The imitated CO2 pneumoperitoneum was established in vitro. After CaSki cells were exposed to CO2 pneumoperitoneum at 0, 7 or 14 mmHg for 1,2, 3, 4 h, the expressions of Survivin mRNA and Bcl-2 protein were examined by RT-PCR and Western blot respectively. The untreated CaSki cells served as control. Results Under 7 or 14 mmHg, the expressions of Survivn and Bcl-2 had an increasing tendency with the exposure time prolongation. Within the same exposure time, the expressions of Survivin and Bcl-2 under 7 mmHg were significantly higher than those under 0 or 14 mmHg or in control group. Conclusion Under certain pressure and within certain exposure time, CO2 pneumoperitoneum could improve anti-apoptotic capability of human cervical carcinoma cells, which may be one reason for the promoting effect of carbon dioxide pneumoperitoneum on malignant cells growth.