吉林省玉米穗腐病病原真菌中镰刀菌毒素的研究

A Study on Production of Three Fusarium Mycotoxins of Corn Ear Rot Pathogenic Fungi in Jilin Province

先前的研究表明，在吉林省田间玉米穗腐病病原真菌中镰刀菌类占80％。本实验采用薄层层析法和气—质联用法对分离自穗腐病的9株镰刀菌进行了脱氧雪腐镰刀菌烯醇、玉米赤霉烯酮和串珠镰刀菌素的定性检测。结果在4株禾谷镰刀菌和一株木贼镰刀菌玉米培养物中检测到脱氧雪腐镰刀菌烯醇；在3株禾谷镰刀菌玉米培养物中检测到了玉米赤霉烯酮；在所有被检菌株中均未测到串珠镰刀菌素。

Previous studies demonstrated 80% corn ear rot pathogenic fungi are Fusari-um spp. in Jilin Province. To investigate if these corn ear rot fungi produce moniliformin, (M-toxin), Zearalenone (ZEA) or/and Deoxynicalenol (DON), extracts from corn culture of nine Fusariun strains (4 F. graminearum strains, 2 F- moniliforme strains, 2F. moniliforme var. subglutinans strains and 1 F. equiseti strain) were analyzed by THL and further by gas chromatograph -mass spectromteter. The results showed that: (1) DON was in corn cultrues of four F. graminearum strains and one F. equiseti strains; (2) Three of four F. gramin-earum strains detectable porduced ZEA; (3) None of the nine Fusarium strains produced M - toxin.