生物微胶囊固定化Candida krusei的研究

Study on Immobilization of Candida krusei by Biocapsule

以一种新型NaCS/PDMDAAC(sodium cellulose sulfate/poly[dimethyl(diallyl)ammonium chloride])生物微胶囊体系包埋耐高渗型产甘油酵母Candia krusei ICM-Y-05。较详细研究了C.krusei在囊内的生长曲线,以及葡萄糖和甘油在囊内外随培养时间变化的情况。结果表明,经生物微胶囊包埋后的细胞在囊内具有二次生长现象。对固定化细胞培养过程中传质系数的测定表明,细胞在囊内的生长对胶囊传质特性有较大的影响,而囊内活细胞数随培养时间的变化并没有减少的趋势,从侧面证明了传质会部分改变囊内细胞生理代谢特性。

Encapsulation of osmotic tolerant yeast Candia krusei ICM-Y-05 by a novel biocapsule system composed of NaCS/PDMDAAC （ sodium cellulose sulfate/ poly [dimethyl（diallyl）ammonium chloride]） was studied in this work. The growth of C. krusei in capsules, and the variation of glucose and glycerol concentration inside/outside capsules associated with the cultivation time were described in detail. The diauxic growth phenomenon of encapsulated cells was observed according to the growth curve. The transfer coefficients of capsules were measured at different culture time. The results showed the obvious effect of cell growth on transfer characteristics of capsules. At the same time, according to the experiment, the number of viable cell in capsules did not decrease with the culture time, which partly verified metabolism properties of cells changing partially due to transfer limitation.