HPLC法测定鸭胚蛋中游离性激素的研究

Determination of Free Sex Hormones in Cherry Vale Duck Embryo by HPLC

研究确立了樱桃谷SM3型鸭胚蛋中的游离性激素17β-雌二醇、丙酸睾酮的测定方法。用甲醇提取鸭胚蛋匀浆液中的17β-雌二醇、丙酸睾酮,再经高效液相色谱仪进行定性、定量分析。该方法线性范围为0.05～3.50μg/mL,标准曲线线性关系良好,其回归方程和相关系数分别为17β-雌二醇y=2278.8x+31.46,R^2= 0.999 9,丙酸睾酮y=925.32x-14.812,R^2=0.9996;检测限为17β-雌二醇2.7ng,丙酸睾酮3.4ng;回收率在89.1%～94.6%之间,相对标准偏差<2.3%。该方法操作简便快捷,有较好的回收率和准确度,适合大量的检测胚胎类保健食品中的游离性激素。

An efficient method for analyzing the sexual hormones of 17β-estradiol and testosterone proprionate that were dissociated in healthy food based on cherry vale duck embryo by high performance liquid chromatography was developed. Sexual hormones such as 17β-estradiol and testosterone proprionate were extracted from homogenized sample with methanol, and then analyzed by HPLC. The line arrange of the method was 0. 05--3. 5~tg/mL,the standard curve linearity relation was fine. The linear equation of 17β-estradiol was y=2 278.8x＋31.46, with correlation coefficient 0. 9999. The linear equation of testosterone proprionate was y=925.32x-14. 812, with correlation coefficient 0. 9996. The limits of detection, 17β-estradiol was 2.7ng and testosterone proprionate was 3.4ng. The average recoveries ranged from 89.1%-94.6%. The relative standard deviation was smaller than 2.3%. Above all, this method was convenient and rapid with the fairly good coefficient of recovery and accurate degree. So, it was suitable to analyze the sexual hormones that were dissociated in healthy food based on embryo.