豹皮樟总黄酮对佐剂性关节炎大鼠炎症免疫功能的影响

Study on anti-inflammatory immunological activity effects of total flavonoids of litsea coreana Leve.Var

目的研究豹皮樟有效部位总黄酮(LCTF)对佐剂性关节炎(AA)大鼠炎症免疫功能的影响,探讨其治疗作用的机制。方法SD大鼠左后足跖皮内注射0·1ml,弗氏完全佐剂复制AA模型;大鼠免疫后12d开始分别灌胃LCTF66·5、133、266mg/kg,连续给药12d。组织块培养法分离培养大鼠滑膜细胞,透射电镜分析滑膜细胞内具分泌功能细胞器的形态学改变;放射免疫测定法检测腹腔巨噬细胞、滑膜细胞分泌IL-1β水平;探讨LCTF作用机制。结果LCTF133、266mg/kg可恢复A、B型滑膜细胞的分泌功能;纠正AA大鼠腹腔巨噬细胞、滑膜细胞过高分泌IL-1β的功能。结论LCTF可恢复AA大鼠滑膜细胞的形态及改善腹腔巨噬细胞、滑膜细胞过高的分泌炎性介质的功能是其治疗AA的途径之一。

Objective To study the anti - inflammatory effect and possible mechanism of total flavonoids of litsea coreana Leve Var. （LCTF） on adjuvant arthritis （AA）. Methods 0. 1 ml of the complete Freund＇s adjuvant was subcutaneously injected into the the left hind feet pads of the SD rats. 12 days after immunization, total flavonoids extracted from litsea coreana Leve. At the dosage of 66. 5.133.266 mg/kg and control drug Tripterygium glycosides 30 mg/kg continuous intragastrice injected administration for 12 days. Synoviocytes were derived from explant culture of tissue fragment, transmission electron microscope was used to analyze the morphological alteration of secretory function organell. Radioimmunoassay was used to detect level of IL-1 βin secretory peritoneal macrophages and synoviocytes. Results LCTF（ 133.266）mg/kg could recovery secretory function of A, B type synoviocytes. LCTF corrected the high level of IL-1 βin secretory peritoneal macrophages and synoviocytes. LCTF has preventive and therapeutic effects on AA rats and could significantly suppresse activity of IL-1β. Conclusion LCTF has effects of anti-inflammatory in AA rats. The anti-inflammatory mechanism of LCTF in AA rats might be related to inhibitory level of IL-1β from peritoneal macrophages and synoviocytes in vivo.