氨氯地平对高血压伴胰岛素抵抗大鼠血清脂联素的影响

Effect of Amlodipine on Serum Adiponectin in Hypertensive and Insulin Resistance Rats

目的:观察氨氯地平对高血压伴胰岛素抵抗动物模型血清脂联素浓度的影响。方法:6周龄的雄性Wistar大鼠随机分为4组(每组10只,均以普通标准饲料)。对照组以普通自来水喂养;果糖组(F组)及氨氯地平治疗组以10/果糖水喂养;氨氯地平组分低剂量组(L组)和高剂量组(H组),果糖水喂养8周后分别以不同浓度的氨氯地平溶液灌胃(1mg·kg-1·d-1和10mg·kg-1·d-1)。胰岛素抵抗指标采用胰岛素敏感性指数(ISI)=-ln(FBG×FINS);尾部测量法测血压;放免法测定血清脂联素的浓度。结果:8周后果糖喂养的F、H、L组大鼠血压、空腹胰岛素水平明显升高,ISI、脂联素明显下降,高血压伴胰岛素抵抗大鼠模型成功建立;氨氯地平干预6周后,L、H组较F组空腹胰岛素水平明显下降,ISI、脂联素明显提高;L、H组间对比无明显差异;多元回归分析表明,体质量和胰岛素敏感性是影响脂联素水平的独立因素。结论:氨氯地平可改善高血压伴胰岛素抵抗大鼠的胰岛素敏感性,其机制可能与提高血清脂联素水平有关。

Objective： To investigate the effect of amlodipine on serum adiponectin level in hypertensive and insulin resistance rat models. Methods： Forty six-week-old male Wistar rats fed with standard diet were randomly divided into 4 groups, control group （tap water）,, fructose group （10% fructose in tap water）, low-dose of amlodipine group（ 1 mg·kg^-1 ·d^-1）and high-dose of amlodipine group （10 mg· kg^-1· d^-1）. The rats in amlodipine groups were treated with 1 mg. kg^-1· d^-1 and 10 mg ·kg^-1·d^-1 of amlodipine after 8 weeks fructose-drinking. Insulin sensitivity index [ISI=-ln （FBGxFINS）] was used to estimate insulin sensitivity. Blood pressure was measured with tail-cuff method. The level of serum adiponectin was determined by radioimmunoassay. Results： The blood pressure and fast insulin （FINS） in 3 fructose groups were significantly increased compared with those of control group at the end of 8 weeks. Insulin sensitivity index and serum adiponectin in 3 fructose groups decreased significantly. The model of rat with hypertension and insulin resistance established successfully. The level of FINS decreased, while ISI and adiponectin increased in amlodipine group compared with those in fructose group after 6-week treatment. However, there were no significant differences between two treatment groups. Multiple regression analysis showed that body mass and ISI were independent factors to adiponectin level. Conclusion： The study suggested that amlodipine can improve insulin sensitivity in hypertensive and insulin resistance rat, partly through increasing serum adiponectin concentration.