林蛙多糖提取方法比较与不同部位多糖含量测定

Comparing Extractions of Temporaria Chensiensis Polysaccharides(TCPS) and determination of the polysaccharides content quantitatively of Rana sylvatica Leconte different parts

目的:对林蛙中多糖(Temporaria Chensiensis Polysaccharides,TCPS)三种常用的提取方法进行比较,并对林蛙不同部位的多糖进行含量测定。方法:采用碱解法、酶解法、碱解酶解法提取林蛙多糖,运用苯酚-硫酸法测定林蛙多糖含量。结果:酶解法林蛙多糖的提取率大于其它两种方法,雌蛙头部的多糖含量最高。

In order to comparing three different extract methods on TCPS and determinate the polysaccharides content quantitatively of Rana sylvatica Leconte different part. Method： alkaline hydrolysis extract method, enzymolysis extract method, alkaline hydrolysis and enzymolysis extract method; Phenol-sulfuric acid assay is applied to determinate the polysaccharides content quantitatively. Result： The yield of enzymolysis extract method is highest. The content of female Rana sylvatica Le conte head is highest.