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HSV1 ICP22对HCMV主要即刻早期启动子结构的效应分析 被引量：1

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摘要 人巨细胞病毒(human cytomegalovirus,HCMV)主要即刻早期(major immediate-early,MIE)启动子具有很强的转录启动能力,其顺式调控元件在这一区域形成多次重复的特殊结构,而这些元件及其组合方式在转录启动过程中的生物学意义尚不清楚.实验发现HSV1即刻早期蛋白ICP22能对多种病毒及细胞启动子及增强子发挥极强的转录抑制作用,利用CAT(氯霉素乙酰转移酶)报道基因系统检测ICP22对各种不同的HCMV即刻早期基因启动子结构元件及突变体转录活性的影响显示:尽管各种元件在ICP22存在的情况下表现出了各自独特的转录效应,但它们组合后形成的不同突变体以及完整的HCMV启动子所表现的转录效率又不是这些元件功能的简单叠加,并且其特定的组合方式能够拮抗ICP22的作用.因此,可以认为,HCMV主要即刻早期基因启动子能以特别的方式与细胞和病毒的转录因子共同调控其下游基因的表达.
作者 罗杰 寸韡 车艳春 王丽春 李卫中 刘龙丁 李琦涵
机构地区 中国医学科学院中国协和医科大学医学生物学研究所
出处 《中国科学（C辑）》 CSCD 北大核心 2007年第3期314-319,共6页 Science in China(Series C)
基金 国家自然科学基金资助项目(批准号:30670094 30570081)
关键词 人巨细胞病毒 ICP22 主要即刻早期基因 启动子
分类号 R373 [医药卫生—病原生物学]
  • 相关文献

参考文献16

  • 1Fields B N, Knipe D M, Howley P M. Fields virology. 3rd ed. Philadelphia: Lippincott-Raven Publishers, 1996. 2493--2523.
  • 2Ho M. Cytomegalovirus: biology and infection. New York: Plenum Publishing Corp, 1991.1--109.
  • 3LaFemina, Hayward G S. Constitutive and retinoic acid-inducible expression of cytomegalovirus immediate-early genes in human teratocarcinoma cells. J Virol, 1986, 58:434--440.
  • 4Ward C M, Stern P L. The human cytomegalovirus immediate-early promoter is transcriptionally active in undifferentiated mouse embryonic stem cells. Stem Cells, 2002, 20(5): 472--475.
  • 5Fortunato E A, Spector D H. Regulation of human cytomegalovirus gene expression. Adv Virus Res, 1999, 54:61--128.
  • 6Meier J L, Stinski M F. Regulation of human cytomegalovirus immediate-early gene expression, lntervirology, 1996, 39(5-6): 331--342.
  • 7Chang Y N, Jeang K T, Lietman T, et al. Structural organization of the spliced immediate-early gene complex that encodes the major acidic nuclear (IE1) and transactivator (IE2) proteins of african green monkey cytomegalovirus. J Biomed Sci, 1995, 2(2): 105-- 130.
  • 8Stinski M F. Cytomegalovirus promoter for expression in mammalian cells. In: Fernandez J M, Hoeffler J P. Gene Expression Systems: using nature for the art of expression. SanDiego: Academic Press, 1999.211--233.
  • 9Isomura H,Tsurumi T, Stinski M F. Role of the proximal enhancer of the major immediate-early promoter in human cytomegalovirus replication. J Virol, 2004, 78(23): 12788--12799.
  • 10Boshart M, Weber F, Jahn G, et al. Very strongEnhancer is located upstream of an immediate early gene of human cytomegalovirus. Cell, 1985, 41:521--530.

二级参考文献19

  • 1Foster T E Melancon J M, Olivier T L, et al. Herpes simplex virus type 1 glycoprotein K and the UL20 protein are interdependent for intracellular trafficking and trans-Golgi network localization [J]. J Virol, 2004, 78:13262-13277.
  • 2Crump C M, Bruun B, Bell S, et al. Alphaherpesvirus glycoprotein M causes the relocalization of plasma membrane proteins [J]. J Gen Virol, 2004, 85 (Pt 12):3517-3527.
  • 3Wysocka J, Herr W. The herpes simplex virus VP16-induced complex: the makings of a regulatory switch [J]. Trends Biochem Sci,2003, 28:294-304.
  • 4Frampton A R Jr, Goins W E Nakano K, et al. HSV trafficking and development of gene therapy vectors with applications in the nervous system [J]. Gene Ther, 2005, 12:891-901.
  • 5CanW LiQH(寸韡 李琦涵).Advanced in analysis of HSV1 immediate early gene [J].国外医学:病毒学分册,2003,10:129-132.
  • 6Song B, Yeh K C, Liu J, et al. Herpes simplex virus gene products required for viral inhibition of expression of G1-phase functions [J].Virology, 2001,290:320-328.
  • 7Advani, S J, Hagglund R, Weichselbaum R R, et al. Posttranslational Processing of Infected Cell Proteins 0 and 4 of Herpes Simplex Virus 1 Is Sequential and Reflects the Subcellular Compartment in Which the Proteins Localize [J]. J Virol, 2001,75:7904-7912.
  • 8Mitchell C, Blaho J A, Roizman B. Casein kinase Ⅱ specifically nucleotidylylates in vitro the amino acid sequence of the protein encoded by the 22 gene of herpes simplex virus 1 [J]. Proc Natl Acad Sci USA, 1994, 91:11864-11868.
  • 9Mitchell C, Blaho J A, McCormick A L, et al. The nucleotidylylation of herpes simplex virus 1 regulatory protein 22 by human casein kinase II [J]. J Biol Chem, 1997, 272:25394-25400.
  • 10Advani S J, Brandimarti R, Weichselbaum R R, et al. The disappearance of cyclins A and B and the increase in activity of the G(2)/M-phase cellular kinase cdc2 in herpes simplex virus 1-infected cells require expression of the alpha22/U(S)1.5 and U(L)13 viral genes [J]. J Virol, 2000, 74:8-15.

同被引文献20

  • 1Mettenleiter T C. Herpesvirus assembly and egress. J Virol, 2002, 76(4): 1537-1547
  • 2Weinheimer S P, Boyd B A, Durham S K, et al. Deletion of the VP16 open reading frame of herpes simplex virus type 1. J Virol, 1992, 66(1): 258-269
  • 3Smibert C A, Johnson D C, Smiley J R. Identification and characterization of the virion-induced host shutoff product of herpes simplex virus gene UL41. J Gen Virol, 1992, 73(Pt 2): 467- 470
  • 4Fields B N, Knipe D M, Howley P M, et al. Fields Virology, 3rd ed. Philadelphia: Lippincott-Raven Publishers, 1996. 2231 -2295
  • 5Heine J W, Honess R W, Cassai E, et al. Proteins specified by herpes simplex virus XⅡ. The virion polypeptides of type 1 strains. J Virol, 1974, 14(3): 640-651
  • 6Elliott G, Mouzakitis G, O'Hare P. VP16 interacts via its activation domain with VP22, a tegument protein of herpes simplex virus, and is relocated to a novel macromolecular assembly in coexpressing cells. J Virol, 1995, 69(12): 7932- 7941
  • 7Elliott G, O'Hare P, Cytoplasm-to-nucleus translocation of a herpesvirus tegument protein during cell division. J Virol, 2000, 74(5): 2131 -2141
  • 8Ren X D, Harms J S, Splitter G A. Bovine herpesvirus 1 tegument protein VP22 interacts with histones, and the carboxyl terminus of V-P22 is required for nuclear localization. J Virol, 2001, 75 (17): 8251 -8258
  • 9Leeuwen H V, Okuwaki M, Hong R, et al. Herpes simplex virus type 1 tegument protein VP22 interacts with TAF-Ⅰ proteins and inhibits nucleosome assembly but not regulation of histone acetylation by INHAT. J Gen Virol, 2003, 84:2501 -2510
  • 10O'Hare P. The virion transactivator of herpes simplex virus. Semin Virol, 1993, 4: 145-155

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二级引证文献2

中国科学（C辑）
2007年 第3期

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