氯胺酮对神经病理性疼痛脊髓背角生长相关蛋白表达的影响

EFFECT OF KETAMINE ON EXPRESSION OF GROWTH ASSOCIATED PROTEIN-43 IN SPINAL DORSAL HORN WITH NEUROPATHIC PAIN

目的:观察氯胺酮对脊神经结扎大鼠脊髓背角生长相关蛋白-43(GAP-43)表达的影响。方法:25只雄性S D大鼠,随机分成5组;假手术组,氯胺酮Ⅰ、Ⅱ、Ⅲ组,生理盐水组。行为学上应用von Frey Hair测定上述各组机械缩足时间变化(n=5),采用免疫组织化学方法测定脊髓背角GAP-43表达的变化(n=5)。结果:氯胺酮组和生理盐水组,1周后均形成神经病理性疼痛模型。腹腔注射不同剂量氯胺酮均可逆转机械性痛觉过敏,氯胺酮组机械缩足时间与生理盐水组比较有显著统计学意义(P(0.001)。各组GAP-43表达随氯胺酮药物浓度增大而含量增加,生理盐水组亦可见GAP-43表达。结论:GAP-43可以阻断神经病理性疼痛中枢敏化的形成,氯胺酮能在很大程度上增强GAP-43的表达,从而起到治疗神经病理性疼痛的作用。

Objectve ： To observe the effects of ketamine （KTM） on expression of growth associated prorein-43 （GAP-43） in spinal dorsal horn of rats with spinal nerve ligation. Methods： Totally 25 male SD rats were randomly divided into 5 groups： sham, KTM Ⅰ, KTM Ⅱ, KTM Ⅲ, and normal saline groups. The mechanical withdrawal time （MWT） was assessed with von Frey filaments （n = 5 ）. The immunochemistry was adopted to detect the change of GAP-43 expression on spinal dorsal horn （ n = 5 ）. Results ： All rats, which received spinal nerve ligation, formed neuropathic pain model in one week. Hyperalgasia was reversed by KTM of different concentration via intraperitoneal administration. There were significant difference （P 〈 0.001 ）, when normal saline group compared with interventioual groups on the MWT. Expression of GAP-43 increased with ascendant dose of ketamine. In addition, GAP-43 reaction product also presented on normal saline group. Conclusion： GAP-43 would contribute to interrupting central sensitization. Expression of GAP-43 can be enhanced with KMT, which had an effort on neuropathic pain.