重组人红细胞生成素、甲泼尼龙对大鼠损伤脊髓中肾上腺髓质素表达的调控

The modulation of erythropoietin and methylprednisolone on the adrenomedullin expression in acute spinal cord injured tissue of rats

目的:观察重组人红细胞生成素(EPO)、甲泼尼龙(MP)在大鼠脊髓损伤中对肾上腺髓质素(AM)表达的调控,探索其作用机制。方法:试验动物随机分为脊髓损伤组(对照组)、EPO治疗组与MP治疗组3组,每组18只,采用Allen′sWD法制成大鼠急性脊髓损伤动物模型。结果:HE染色EPO治疗组与MP治疗组病理变化轻于对照组。免疫组化染色3组中均有AM的表达;EPO组及MP组AM表达3d、6d高于对照组(P<0.01),MP组AM表达3d高于EPO组(P<0.05),6d、11d两组差异无显著性意义。神经功能评分EPO组、MP组高于对照组,MP组3d高于EPO组(P<0.05),6d、11d两组差异无显著性意义。结论:EPO、MP能上调AM的表达,促进神经功能恢复,这可能是MP、EPO在损伤脊髓中的保护作用机制之一。

Objective：To discuss the modulation of erythropoietin （EPO） and methylprednisolone （MP） on the adrenomedullin expression in acute spinal cord injured tissue of rats and explore the mechanism. Method：The rats were divided into three groups by randomization：Control, EPO cure and MP cure groups. There were 18 rats in each group. SD rat model of acute spinal cord injury were established by Allen＇s weight drop （WD）. Result： The HE staining pathological changes in MP and EPO cure groups were mitigated than control group. Expression of AM was found in the spinal cord tissues of the three groups. The expression level of AM in EPO and MP cure groups was obviously higher than in control group with significant difference （P〈0.01） on the 3rd and 6th day. The expression level of AM in MP cure group was higher than that in EPO cure group on the 3rd day （P〈0.05） and had no significant difference on 6th and l lth day. The scores of nervous condition in MP cure and EPO cure groups were higher than that of control group. The MP cure group＇s score was higher than EPO cure group＇s on the 3rd day （P〈0.05）. It had no significant difference on the 6th and lhh day. Conclusion：Expression of AM can increased after treatment of MP and EPO. Maybe it is one of the protective mechanisms of MP and EPO in SCI.